論文 - 詳細
| RRC ID | 83095 |
|---|---|
| 著者 | Zecca A, Dyballa S, Voltes A, Bradley R, Pujades C. |
| タイトル | The Order and Place of Neuronal Differentiation Establish the Topography of Sensory Projections and the Entry Points within the Hindbrain. |
| ジャーナル | J Neurosci |
| Abstract |
Establishing topographical maps of the external world is an important but still poorly understood feature of the vertebrate sensory system. To study the selective innervation of hindbrain regions by sensory afferents in the zebrafish embryo, we mapped the fine-grained topographical representation of sensory projections at the central level by specific photoconversion of sensory neurons. Sensory ganglia located anteriorly project more medially than do ganglia located posteriorly, and this relates to the order of sensory ganglion differentiation. By single-plane illumination microscopy (SPIM) in vivo imaging, we show that (1) the sequence of arrival of cranial ganglion inputs predicts the topography of central projections, and (2) delaminated neuroblasts differentiate in close contact with the neural tube, and they never loose contact with the neural ectoderm. Afferent entrance points are established by plasma membrane interactions between primary differentiated peripheral sensory neurons and neural tube border cells with the cooperation of neural crest cells. These first contacts remain during ensuing morphological growth to establish pioneer axons. Neural crest cells and repulsive slit1/robo2 signals then guide axons from later-differentiating neurons toward the neural tube. Thus, this study proposes a new model by which the topographical representation of cranial sensory ganglia is established by entrance order, with the entry points determined by cell contact between the sensory ganglion cell bodies and the hindbrain. |
| 巻・号 | 35(19) |
| ページ | 7475-86 |
| 公開日 | 2015-5-13 |
| DOI | 10.1523/JNEUROSCI.3743-14.2015 |
| PII | 35/19/7475 |
| PMID | 25972174 |
| PMC | PMC6705443 |
| MeSH | Afferent Pathways / drug effects Afferent Pathways / physiology* Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Brain Mapping* Cell Differentiation / genetics Cell Differentiation / physiology* Chemokine CXCL12 / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Embryo, Nonmammalian Enzyme Inhibitors / pharmacology Female Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Isoxazoles / pharmacology LIM-Homeodomain Proteins / genetics LIM-Homeodomain Proteins / metabolism Leflunomide Male Morpholinos / pharmacology Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neural Tube / cytology Rhombencephalon / anatomy & histology* Rhombencephalon / drug effects Rhombencephalon / embryology Sensory Receptor Cells / drug effects Sensory Receptor Cells / physiology* Signal Transduction / genetics Signal Transduction / physiology Transcription Factors / genetics Transcription Factors / metabolism Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 5.674 |
| リソース情報 | |
| ゼブラフィッシュ | hspGFF53A |