Reference - Detail
| RRC ID | 83414 |
|---|---|
| Author | Kanuka H, Sawamoto K, Inohara N, Matsuno K, Okano H, Miura M. |
| Title | Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator. |
| Journal | Mol Cell |
| Abstract |
We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution. |
| Volume | 4(5) |
| Pages | 757-69 |
| Published | 1999-11-1 |
| DOI | 10.1016/s1097-2765(00)80386-x |
| PII | S1097-2765(00)80386-X |
| PMID | 10619023 |
| MeSH | Alternative Splicing Amino Acid Sequence Animals Apoptosis* Apoptotic Protease-Activating Factor 1 Base Sequence Brain / cytology Brain / embryology Brain / metabolism Caenorhabditis elegans Proteins* Calcium-Binding Proteins / chemistry* Calcium-Binding Proteins / genetics Caspases / chemistry Caspases / metabolism* Cell Line Cloning, Molecular Cytochrome c Group / metabolism Drosophila Proteins* Drosophila melanogaster / cytology* Drosophila melanogaster / embryology Drosophila melanogaster / enzymology Drosophila melanogaster / genetics Enzyme Activation Evolution, Molecular Eye / cytology Eye / metabolism Helminth Proteins / chemistry* Helminth Proteins / genetics Larva / cytology Larva / metabolism Molecular Sequence Data Mutation / genetics Peptides / genetics Peptides / physiology Protein Binding Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / metabolism Proteins / chemistry* Proteins / genetics RNA, Messenger / analysis RNA, Messenger / genetics Sequence Homology, Amino Acid |
| IF | 15.584 |
| Resource | |
| Drosophila | DGRC#119558 |