論文 - 詳細
| RRC ID | 86760 |
|---|---|
| 著者 | Hasan SS, Tsaryk R, Lange M, Wisniewski L, Moore JC, Lawson ND, Wojciechowska K, Schnittler H, Siekmann AF. |
| タイトル | Endothelial Notch signalling limits angiogenesis via control of artery formation. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Angiogenic sprouting needs to be tightly controlled. It has been suggested that the Notch ligand dll4 expressed in leading tip cells restricts angiogenesis by activating Notch signalling in trailing stalk cells. Here, we show using live imaging in zebrafish that activation of Notch signalling is rather required in tip cells. Notch activation initially triggers expression of the chemokine receptor cxcr4a. This allows for proper tip cell migration and connection to the pre-existing arterial circulation, ultimately establishing functional arterial-venous blood flow patterns. Subsequently, Notch signalling reduces cxcr4a expression, thereby preventing excessive blood vessel growth. Finally, we find that Notch signalling is dispensable for limiting blood vessel growth during venous plexus formation that does not generate arteries. Together, these findings link the role of Notch signalling in limiting angiogenesis to its role during artery formation and provide a framework for our understanding of the mechanisms underlying blood vessel network expansion and maturation. |
| 巻・号 | 19(8) |
| ページ | 928-940 |
| 公開日 | 2017-8-1 |
| DOI | 10.1038/ncb3574 |
| PII | ncb3574 |
| PMID | 28714969 |
| PMC | PMC5534340 |
| MeSH | Animals Animals, Genetically Modified Arteries / cytology Arteries / metabolism* Cell Movement Cells, Cultured Endothelial Cells / metabolism* Gene Expression Regulation, Developmental Genotype Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Human Umbilical Vein Endothelial Cells / metabolism Humans Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Microscopy, Fluorescence Microscopy, Video Neovascularization, Physiologic* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Phenotype Receptor, Notch1 / genetics Receptor, Notch1 / metabolism* Receptors, CXCR4 / genetics Receptors, CXCR4 / metabolism Signal Transduction Time Factors Time-Lapse Imaging Transfection Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 20.042 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |