| Author |
Nam S, Kim Y, Lee MJ, Kim YB, Yun JU, Jung MJ, Song HS, Lee SH, Kim SJ, Whon TW.
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| Abstract |
A novel Gram-stain-positive, non-spore-forming, non-motile and rod-shaped bacterium, designated strain CBA3109T, was isolated from jogae-jeotgal (fermented clam), a traditional Korean fermented seafood. Strain CBA3109T showed growth at 10-30 °C (optimum, 25 °C) and pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-15% (w/v) NaCl (optimum, 5%). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CBA3109T belonged to the genus Brevibacterium, with the highest similarities to Brevibacterium aurantiacum NCDO 739T (98.26%) and B. antiquum VKM Ac-2118T (98.14%). Strain CBA3109T contained MK-8(H2) as the major menaquinone. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major fatty acids (>10%) were anteiso-C15 : 0 and anteiso-C17 : 0. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values between strain CBA3109T and the closest species were 86.2-87.0% and 31.1-33.0 %, respectively. The DNA G+C content of strain CBA3109T was 62.7%. Based on the morphological, phylogenetic, chemotaxonomic and genotypic data, strain CBA3109T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium koreense sp. nov. is proposed. The type strain is CBA3109T (= KACC 23387T = DSM 117564T).
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