Reference - Detail
|Author||Yamasaki Y, Nishida Y.|
|Title||Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila.|
|Journal||Dev Growth Differ|
Mi-2, the central component of the nucleosome remodeling and histone deacetylation (NuRD) complex, is known as an SNF2-type ATP-dependent nucleosome remodeling factor. No morphological mutant phenotype of Drosophila Mi-2 (dMi-2) had been reported previously; however, we found that rare escapers develop into adult flies showing an extra bristle phenotype. The dMi-2 enhanced the phenotype of ac(Hw49c), which is a dominant gain-of-function allele of achaete (ac) and produces extra bristles. Consistent with these observations, the ac-expressing proneural clusters were expanded, and extra sensory organ precursors (SOP) were formed in the dMi-2 mutant wing discs. Immunostaining of polytene chromosomes showed that dMi-2 binds to the ac locus, and dMi-2 and acetylated hisotones distribute on polytene chromosomes in a mutually exclusive manner. The chromatin immunoprecipitation assay of the wing imaginal disc also demonstrated a binding of dMi-2 on the ac locus. These results suggest that the Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation.
|MeSH||Acetylation Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Alleles Animals Autoantigens / genetics Autoantigens / metabolism* Chromatin Immunoprecipitation Drosophila / genetics* Drosophila / growth & development Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental / genetics Histone Deacetylases / genetics Histone Deacetylases / metabolism Histones / metabolism Immunohistochemistry Mi-2 Nucleosome Remodeling and Deacetylase Complex Mutation / genetics Nucleosomes / genetics Nucleosomes / metabolism Phenotype Protein Binding Sense Organs / growth & development Sense Organs / metabolism* Wings, Animal / anatomy & histology Wings, Animal / growth & development Wings, Animal / metabolism|
|WOS Category||DEVELOPMENTAL BIOLOGY CELL BIOLOGY|