論文 - 詳細
| RRC ID | 11582 |
|---|---|
| 著者 | Yamasaki Y, Nishida Y. |
| タイトル | Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila. |
| ジャーナル | Dev Growth Differ |
| Abstract |
Mi-2, the central component of the nucleosome remodeling and histone deacetylation (NuRD) complex, is known as an SNF2-type ATP-dependent nucleosome remodeling factor. No morphological mutant phenotype of Drosophila Mi-2 (dMi-2) had been reported previously; however, we found that rare escapers develop into adult flies showing an extra bristle phenotype. The dMi-2 enhanced the phenotype of ac(Hw49c), which is a dominant gain-of-function allele of achaete (ac) and produces extra bristles. Consistent with these observations, the ac-expressing proneural clusters were expanded, and extra sensory organ precursors (SOP) were formed in the dMi-2 mutant wing discs. Immunostaining of polytene chromosomes showed that dMi-2 binds to the ac locus, and dMi-2 and acetylated hisotones distribute on polytene chromosomes in a mutually exclusive manner. The chromatin immunoprecipitation assay of the wing imaginal disc also demonstrated a binding of dMi-2 on the ac locus. These results suggest that the Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation. |
| 巻・号 | 48(7) |
| ページ | 411-8 |
| 公開日 | 2006-9-1 |
| DOI | 10.1111/j.1440-169X.2006.00880.x |
| PII | DGD880 |
| PMID | 16961588 |
| MeSH | Acetylation Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Alleles Animals Autoantigens / genetics Autoantigens / metabolism* Chromatin Immunoprecipitation Drosophila / genetics* Drosophila / growth & development Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental / genetics Histone Deacetylases / genetics Histone Deacetylases / metabolism Histones / metabolism Immunohistochemistry Mi-2 Nucleosome Remodeling and Deacetylase Complex Mutation / genetics Nucleosomes / genetics Nucleosomes / metabolism Phenotype Protein Binding Sense Organs / growth & development Sense Organs / metabolism* Wings, Animal / anatomy & histology Wings, Animal / growth & development Wings, Animal / metabolism |
| IF | 1.723 |
| 引用数 | 11 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | DGRC#109812 DGRC#104384 |