Reference - Detail
| RRC ID | 1344 |
|---|---|
| Author | Kawano T, Horiguchi-Yamada J, Saito S, Iwase S, Furukawa Y, Kano Y, Yamada H. |
| Title | Ectopic cyclin D1 expression blocks STI571-induced erythroid differentiation of K562 cells. |
| Journal | Leuk Res |
| Abstract |
Bcr-Abl tyrosine kinase inhibitor induces apoptosis and erythroid differentiation of K562 cells. During this erythroid differentiation, c-Myc and cyclin D1 transcripts are transiently downregulated. Accordingly, we studied the effect of cyclin D1 overexpression on erythroid differentiation. After treatment with 250 nM STI571, 90% of K562 and 25% of K562/D1 cells underwent erythroid differentiation. The basal expression of glycophorin A in K562/D1 cells was markedly diminished compared with that by parental cells. STI571 treatment failed to induce glycophorin A expression in K562/D1 cells. During STI571 treatment, ERK activity was downregulated in parental cells, while it was constantly activated in K562/D1 cells. These results suggest that ectopic expression of cyclin D1 causes the resistance of K562 cells to erythroid differentiation by modulating ERK regulation. |
| Volume | 28(6) |
| Pages | 623-9 |
| Published | 2004-6-1 |
| DOI | 10.1016/j.leukres.2003.10.022 |
| PII | S0145212603003527 |
| PMID | 15120940 |
| MeSH | Benzamides Benzoquinones Cell Differentiation / drug effects* Cyclin D1 / metabolism* Down-Regulation Drug Resistance, Neoplasm Enzyme Inhibitors / pharmacology* Erythroid Precursor Cells / drug effects* Erythroid Precursor Cells / pathology Glycophorins / metabolism Humans Imatinib Mesylate K562 Cells Lactams, Macrocyclic Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases / metabolism Mitogen-Activated Protein Kinases / metabolism Phosphorylation Piperazines / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors Pyrimidines / pharmacology* Quinones / pharmacology Rifabutin / analogs & derivatives Signal Transduction Transfection |
| IF | 2.214 |
| Times Cited | 15 |
| WOS Category | HEMATOLOGY ONCOLOGY |
| Resource | |
| Human and Animal Cells | K562(RCB0027) |