RRC ID 15867
Author Cai Y, Yang J, Pang H, Kitahara M.
Title Lactococcus fujiensis sp. nov., a lactic acid bacterium isolated from vegetable matter.
Journal Int J Syst Evol Microbiol
Abstract Three strains of lactic acid bacteria, designated NJ 317(T), NJ 414 and NJ 415, were isolated from the outer leaves of Chinese cabbages (Brassica rapa L. var. glabra Regel) and characterized taxonomically. The strains were gram-reaction-positive, catalase-negative, facultatively anaerobic cocci that did not produce gas from glucose and formed L-lactic acid. The major fatty acids were C(18 : 1)ω9c, C(16 : 0), C(14 : 0) and summed feature 10. Morphological, physiological and phylogenetic data indicated that the strains belonged to the genus Lactococcus. These strains shared similar phenotypic characteristics and exhibited DNA relatedness values >96.6 % to each other, indicating that they represent a single species. The DNA G+C contents of the three strains were 42.1-42.5 mol%. 16S rRNA gene sequences of the novel strains were determined and aligned with those of other species of the genus Lactococcus. On the basis of phylogenetic analysis the three strains grouped with other members of the genus Lactococcus. Lactococcus lactis and Lactococcus garvieae were the most closely related species, sharing a sequence similarity value of 94.4 % with the three strains. Ribotyping patterns, however, revealed that these strains were well-separated from reference strains of species of the genus Lactococcus and DNA-DNA hybridization studies indicated that the novel strains had low levels (<20.2 %) of DNA relatedness with reference strains of L. lactis, L. garvieae and other type strains of previously described species, showing that they represent a different species. Based on this evidence, strains NJ 317(T), NJ 414 and NJ 415 represent a novel species of the genus Lactococcus, for which the name Lactococcus fujiensis sp. nov. is proposed. The type strain is NJ 317(T) ( = JCM16395(T)  = CGMCC 1.10453(T)).
Volume 61(Pt 7)
Pages 1590-1594
Published 2011-7-1
DOI 10.1099/ijs.0.025130-0
PMID 20675438
MeSH Bacterial Typing Techniques Base Composition Brassica / microbiology* DNA, Bacterial / genetics Fatty Acids / chemistry Japan Lactic Acid Lactococcus / classification* Lactococcus / genetics Lactococcus / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Ribotyping Sequence Analysis, DNA
IF 2.415
Times Cited 21
General Microbes JCM 1180 JCM 5706 JCM 5805 JCM 10343 JCM 11055 JCM 11056 JCM 16167 JCM16395 JCM 17100