論文 - 詳細
| RRC ID | 18918 |
|---|---|
| 著者 | Zhang Y, Huang Q, Cheng JC, Nishi Y, Yanase T, Huang HF, Leung PC. |
| タイトル | Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells. |
| ジャーナル | Reprod Biol Endocrinol |
| Abstract |
BACKGROUND:Homeobox (HOX) genes encode transcription factors, which regulate cell proliferation, differentiation, adhesion, and migration. The deregulation of HOX genes is frequently associated with human reproductive system disorders. However, knowledge regarding the role of HOX genes in human granulosa cells is limited. METHODS:To determine the role of HOXA7 in the regulation and associated mechanisms of cell proliferation in human granulosa cells, HOXA7 and epidermal growth factor receptor (EGFR) expressions were examined in primary granulosa cells (hGCs), an immortalized human granulosa cell line, SVOG, and a granulosa tumor cell line, KGN, by real-time PCR and Western blotting. To manipulate the expression of HOXA7, the HOXA7 specific siRNA was used to knockdown HOXA7 in KGN. Conversely, HOXA7 was overexpressed in SVOG by transfection with the pcDNA3.1-HOAX7 vector. Cell proliferation was measured by the MTT assay. RESULTS:Our results show that HOXA7 and EGFR were overexpressed in KGN cells compared to hGCs and SVOG cells. Knockdown of HOXA7 in KGN cells significantly decreased cell proliferation and EGFR expression. Overexpression of HOXA7 in SVOG cells significantly promoted cell growth and EGFR expression. Moreover, the EGF-induced KGN proliferation was abrogated, and the activation of downstream signaling was diminished when HOXA7 was knocked down. Overexpression of HOXA7 in SVOG cells had an opposite effect. CONCLUSIONS:Our present study reveals a novel mechanistic role for HOXA7 in modulating granulosa cell proliferation via the regulation of EGFR. This finding contributes to the knowledge of the pro-proliferation effect of HOXA7 in granulosa cell growth and differentiation. |
| 巻・号 | 8 |
| ページ | 61 |
| 公開日 | 2010-6-14 |
| DOI | 10.1186/1477-7827-8-61 |
| PII | 1477-7827-8-61 |
| PMID | 20540809 |
| PMC | PMC2904782 |
| MeSH | Cell Differentiation / drug effects Cell Differentiation / genetics Cell Proliferation* / drug effects Cells, Cultured Enzyme Activation ErbB Receptors / genetics* ErbB Receptors / metabolism Female Granulosa Cells / drug effects Granulosa Cells / metabolism* Homeodomain Proteins / antagonists & inhibitors Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Homeodomain Proteins / physiology* Humans MAP Kinase Signaling System / drug effects MAP Kinase Signaling System / genetics Oncogene Protein v-akt / metabolism Phosphatidylinositol 3-Kinases / metabolism RNA, Small Interfering / pharmacology Up-Regulation / drug effects Up-Regulation / genetics Up-Regulation / physiology |
| IF | 3.235 |
| 引用数 | 14 |
| WOS 分野 | REPRODUCTIVE BIOLOGY ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |