RRC ID 19130
Author Mukaiyama H, Kodera M, Tanaka N, Takegawa K.
Title N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe.
Journal Appl. Microbiol. Biotechnol.
Abstract In eukaryotic cells, aberrant proteins generated in the endoplasmic reticulum (ER) are degraded by the ER-associated degradation (ERAD) pathway. Here, we report on the ERAD pathway of the fission yeast Schizosaccharomyces pombe. We constructed and expressed Saccharomyces cerevisiae wild-type CPY (ScCPY) and CPY-G255R mutant (ScCPY*) in S. pombe. While ScCPY was glycosylated and efficiently transported to the vacuoles in S. pombe, ScCPY* was retained in the ER and was not processed to the matured form in these cells. Cycloheximide chase experiments revealed that ScCPY* was rapidly degraded in S. pombe, and its degradation depended on Hrd1p and Ubc7p homologs. We also found that Mnl1p and Yos9p, proteins that are essential for ERAD in S. cerevisiae, were not required for ScCPY* degradation in S. pombe. Moreover, the null-glycosylation mutant of ScCPY, CPY*0000, was rapidly degraded by the ERAD pathway. These results suggested that N-linked oligosaccharides are not important for the recognition of luminal proteins for ERAD in S. pombe cells.
Volume 93(4)
Pages 1609-18
Published 2012-2
DOI 10.1007/s00253-011-3662-z
PMID 22083275
MeSH Amino Acid Substitution Carboxypeptidases / genetics* Carboxypeptidases / metabolism* Endoplasmic Reticulum / metabolism Mutant Proteins / genetics Mutant Proteins / metabolism Mutation, Missense Polysaccharides / metabolism* Protein Processing, Post-Translational Proteolysis* Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism Ubiquitin-Conjugating Enzymes / metabolism Ubiquitin-Protein Ligases / metabolism
IF 3.42
Times Cited 0
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Yeast FY18625