RRC ID 25675
Author Mizuno N, Shitsukawa N, Hosogi N, Park P, Takumi S.
Title Autoimmune response and repression of mitotic cell division occur in inter-specific crosses between tetraploid wheat and Aegilops tauschii Coss. that show low temperature-induced hybrid necrosis.
Journal Plant J.
Abstract Common wheat is an allohexaploid species originating from a naturally occurring inter-specific cross between tetraploid wheat and the diploid wild wheat Aegilops tauschii Coss. Artificial allopolyploidization can produce synthetic hexaploid wheat. However, synthetic triploid hybrids show four types of hybrid growth abnormalities: type II and III hybrid necrosis, hybrid chlorosis, and severe growth abortion. Of these hybrid abnormalities, type II necrosis is induced by low temperature. Under low temperature, elongation of stems and expansion of new leaves is repressed in type II necrosis lines, which later exhibit necrotic symptoms. Here, we characterize type II necrosis in detail. Comparative transcriptome analysis showed that a number of defense-related genes were highly up-regulated in seedling leaves that showed type II necrosis. Transmission electron microscopy revealed extensive cell death in the leaves under low-temperature conditions, accompanied by abundant generation of reactive oxygen species. In addition, down-regulation of cell cycle-related genes was observed in shoot apices of type II necrosis lines under low-temperature conditions. Quantitative RT-PCR and in situ hybridization showed repression of accumulation of histone H4 transcripts in the shoot apical meristem of type II necrosis lines. These results strongly suggest that an autoimmune response-like reaction and repression of cell division in the shoot apical meristem are associated with the abnormal growth phenotype in type II necrosis lines.
Volume 68(1)
Pages 114-28
Published 2011-10
DOI 10.1111/j.1365-313X.2011.04667.x
PMID 21645146
MeSH Autoimmunity / genetics* Cell Death Chromosome Mapping Cold Temperature Down-Regulation Gene Expression Regulation, Plant Genome, Plant / genetics Histones / genetics Hybridization, Genetic / physiology Meristem / genetics Meristem / growth & development Meristem / physiology Meristem / ultrastructure Mitosis / genetics* Mitosis / immunology Necrosis / genetics Phenotype Plant Leaves / genetics Plant Leaves / growth & development Plant Leaves / physiology Plant Leaves / ultrastructure Plant Proteins / genetics Plant Proteins / metabolism Plant Shoots / genetics Plant Shoots / growth & development Plant Shoots / physiology Plant Shoots / ultrastructure Poaceae / genetics Poaceae / physiology* Poaceae / ultrastructure Polyploidy RNA, Messenger / genetics RNA, Plant / genetics Reactive Oxygen Species / metabolism Transcriptome* Triticum / genetics Triticum / physiology* Triticum / ultrastructure
IF 5.775
Times Cited 17
WOS Category PLANT SCIENCES
Resource
Wheat KU-2059 KU-2025 KU-2075 KU-2828 KU-2159 KU-2012 KU-2145