| RRC ID |
28693
|
| Author |
Smialowska A, Djupedal I, Wang J, Kylsten P, Swoboda P, Ekwall K.
|
| Title |
RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.
|
| Volume |
444(2)
|
| Pages |
254-9
|
| Published |
2014-2-7
|
| DOI |
10.1016/j.bbrc.2014.01.057
|
| PII |
S0006-291X(14)00081-3
|
| PMID |
24462781
|
| MeSH |
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Blotting, Northern
Blotting, Western
Endoribonucleases / genetics
Endoribonucleases / metabolism
Gene Expression Regulation, Fungal*
Microscopy, Fluorescence
Mutation
Oligonucleotide Array Sequence Analysis
RNA Interference*
RNA, Antisense / genetics
RNA, Fungal / genetics
RNA, Messenger / genetics
RNA, Small Interfering / genetics
RNA, Small Untranslated / genetics
RNA-Dependent RNA Polymerase / genetics
RNA-Dependent RNA Polymerase / metabolism
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
Transcriptome / genetics*
|
| IF |
2.985
|
| Times Cited |
11
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
S. pombe YFP-FLAG-His
SpYFH23B11 (SPW089235)
SpYFH22C07 (SPW088855)
SpYFH46F08 (SPW098528)
SpYFH40H10 (SPW096178)
SpYFH38C10 (SPW095258)
SpYFH04F01 (SPW081721)
SpYFH39B05 (SPW095629)
SpYFH31D08 (SPW092480). |
| Yeast |
|
