論文 - 詳細
| RRC ID | 29105 |
|---|---|
| 著者 | Meili R, Ellsworth C, Lee S, Reddy TB, Ma H, Firtel RA. |
| タイトル | Chemoattractant-mediated transient activation and membrane localization of Akt/PKB is required for efficient chemotaxis to cAMP in Dictyostelium. |
| ジャーナル | EMBO J |
| Abstract |
Chemotaxis-competent cells respond to a variety of ligands by activating second messenger pathways leading to changes in the actin/myosin cytoskeleton and directed cell movement. We demonstrate that Dictyostelium Akt/PKB, a homologue of mammalian Akt/PKB, is very rapidly and transiently activated by the chemoattractant cAMP. This activation takes place through G protein-coupled chemoattractant receptors via a pathway that requires homologues of mammalian p110 phosphoinositide-3 kinase. pkbA null cells exhibit aggregation-stage defects that include aberrant chemotaxis, a failure to polarize properly in a chemoattractant gradient and aggregation at low densities. Mechanistically, we demonstrate that the PH domain of Akt/PKB fused to GFP transiently translocates to the plasma membrane in response to cAMP with kinetics similar to those of Akt/PKB kinase activation and is localized to the leading edge of chemotaxing cells in vivo. Our results indicate Akt/PKB is part of the regulatory network required for sensing and responding to the chemoattractant gradient that mediates chemotaxis and aggregation. |
| 巻・号 | 18(8) |
| ページ | 2092-105 |
| 公開日 | 1999-4-15 |
| DOI | 10.1093/emboj/18.8.2092 |
| PMID | 10205164 |
| PMC | PMC1171294 |
| MeSH | Amino Acid Sequence Animals Base Sequence Biological Transport Cell Membrane / metabolism Chemotactic Factors / pharmacology* Chemotaxis* Cyclic AMP / pharmacology* DNA Primers Dictyostelium / drug effects* Dictyostelium / metabolism Enzyme Activation Green Fluorescent Proteins Luminescent Proteins / genetics Molecular Sequence Data Phosphatidylinositol 3-Kinases / metabolism Protein Serine-Threonine Kinases* Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-akt Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 9.889 |
| 引用数 | 356 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 細胞性粘菌 | S00335 |