RRC ID 30937
Author Sato K, Sato M, Nakano A.
Title Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes.
Journal Mol Biol Cell
Abstract The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) membrane proteins such as Sec12p and Sec71p to the ER. We demonstrate here that the transmembrane domain (TMD) of Sec71p, a type-III membrane protein, contains an ER localization signal, which is required for physical recognition by Rer1p. The Sec71TMD-GFP fusion protein is efficiently retrieved to the ER by Rer1p. The structural feature of this TMD signal turns out to be the spatial location of polar residues flanking the highly hydrophobic core sequence but not the whole length of the TMD. On the Rer1p side, Tyr152 residue in the 4th TMD is important for the recognition of Sec12p but not Sec71p, suggesting that Rer1p interacts with its ligands at least in two modes. Sec71TMD-GFP expressed in the Deltarer1 mutant cells is mislocalized from the ER to the lumen of vacuoles via the multivesicular body (MVB) sorting pathway. In this case, not only the presence of polar residues in the Sec71TMD but also the length of the TMD is critical for the MVB sorting. Thus, the Rer1p-dependent ER retrieval and the MVB sorting in late endosomes both watch polar residues in the TMD but in a different manner.
Volume 14(9)
Pages 3605-16
Published 2003-9-1
DOI 10.1091/mbc.e02-12-0777
PII E02-12-0777
PMID 12972550
PMC PMC196553
MeSH Amino Acid Sequence Cell Compartmentation Endoplasmic Reticulum / metabolism Endoplasmic Reticulum / physiology Fungal Proteins / genetics Fungal Proteins / metabolism* Fungal Proteins / physiology Green Fluorescent Proteins Guanine Nucleotide Exchange Factors Luminescent Proteins Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Membrane Glycoproteins / physiology Membrane Proteins / genetics Membrane Proteins / metabolism* Membrane Proteins / physiology Models, Molecular Molecular Sequence Data Mutation Protein Binding / physiology Protein Structure, Tertiary / genetics Protein Transport / physiology Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae / physiology Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Saccharomyces cerevisiae Proteins / physiology Sequence Analysis, Protein Vesicular Transport Proteins
IF 3.905
Times Cited 80
WOS Category CELL BIOLOGY
Resource
DNA material 316-GFP-SEC12 (RDB08651) 316-M71W71 (RDB08633) 316-M71WWS (RDB08630) 316-MW71WS (RDB08631) 316-MW71WS/1L (RDB08638) 316-MW71WS/2L (RDB08639) 316-MW71WS/2L delta LV (RDB08642) 316-MW71WS/3L (RDB08640) 316-MW71WS/4L (RDB08641) 316-MW71WS/delta L (RDB08637) 316-MW71WS/LS (RDB08634) 316-MW71WS/SA (RDB08648) 316-MW71WS/SL (RDB08636) 316-MW71WS/SQ (RDB08647) 316-MW71WS/SY (RDB08646) 316-MW71WS/YA (RDB08645) 316-MW71WS/YL (RDB08635) 316-MW71WS/YQ (RDB08644) 316-MW71WS/YS (RDB08643) 316-MWW71 (RDB08632) 316-MWWWK (RDB08628) 316-MWWWS (RDB08629) pQR316-SEC71-GFP (RDB08598) pQR316-SEC71TMD-GFP (RDB08653) pQR316-SEC71TMD-GFP/1L (RDB08610) pQR316-SEC71TMD-GFP/2L (RDB08611) pQR316-SEC71TMD-GFP/2L delta LV (RDB08614) pQR316-SEC71TMD-GFP/3L (RDB08612) pQR316-SEC71TMD-GFP/4L (RDB08613) pQR316-SEC71TMD-GFP/LS (RDB08608) pQR316-SEC71TMD-GFP/SL (RDB08609) pQR326-SEC71-GFP (RDB08599) pRS306-SEC71TMD-GFP (RDB08652) pRS306-SEC71TMD-GFP/1L (RDB08603) pRS306-SEC71TMD-GFP/2L (RDB08604) pRS306-SEC71TMD-GFP/2L delta LV (RDB08607) pRS306-SEC71TMD-GFP/3L (RDB08605) pRS306-SEC71TMD-GFP/4L (RDB08606) pRS306-SEC71TMD-GFP/LS (RDB08600) pRS306-SEC71TMD-GFP/SL (RDB08601) pRS306-SEC71TMD-GFP/YL (RDB08602) pRS316MS63F (RDB08583) pRS316MS71H (RDB08581) pSKY54-SEC12 (RDB08657) pSKY5wRER1-0 (RDB08585) pSKY5wRER1N76L (RDB08587) pSKY5wRER1Q77L (RDB08588) pSKY5wRER1S131L (RDB08591) pSKY5wRER1S135L (RDB08592) pSKY5wRER1S138L (RDB08593) pSKY5wRER1T128L (RDB08590) pSKY5wRER1T160L (RDB08595) pSKY5wRER1T83L (RDB08589) pSKY5wRER1Y152L (RDB08594) pSKY5wRER1Y68L (RDB08586) pYO326MS63F (RDB08584) pYO326MS71H (RDB08582) SEC12-MF(alpha)1 (pSKY1) (RDB08580)