論文 - 詳細
| RRC ID | 323 |
|---|---|
| 著者 | Kamimura K, Rhodes JM, Ueda R, McNeely M, Shukla D, Kimata K, Spear PG, Shworak NW, Nakato H. |
| タイトル | Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase. |
| ジャーナル | J Cell Biol |
| Abstract |
Heparan sulfate (HS) regulates the activity of various ligands and is involved in molecular recognition events on the cell surface and in the extracellular matrix. Specific binding of HS to different ligand proteins depends on the sulfation pattern of HS. For example, the interaction between antithrombin and a particular 3-O sulfated HS motif is thought to modulate blood coagulation. However, a recent study of mice defective for this modification suggested that 3-O sulfation plays other biological roles. Here, we show that Drosophila melanogaster HS 3-O sulfotransferase-b (Hs3st-B), which catalyzes HS 3-O sulfation, is a novel component of the Notch pathway. Reduction of Hs3st-B function by transgenic RNA interference compromised Notch signaling, producing neurogenic phenotypes. We also show that levels of Notch protein on the cell surface were markedly decreased by loss of Hs3st-B. These findings suggest that Hs3st-B is involved in Notch signaling by affecting stability or intracellular trafficking of Notch protein. |
| 巻・号 | 166(7) |
| ページ | 1069-79 |
| 公開日 | 2004-9-27 |
| DOI | 10.1083/jcb.200403077 |
| PII | jcb.200403077 |
| PMID | 15452147 |
| PMC | PMC2172002 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence / genetics Cell Membrane / genetics Cell Membrane / metabolism DNA, Complementary / analysis DNA, Complementary / genetics Down-Regulation / genetics Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Extracellular Matrix / genetics Extracellular Matrix / metabolism Gene Expression Regulation, Developmental / genetics Heparitin Sulfate / metabolism* Intracellular Membranes / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Phenotype Protein Binding / genetics Protein Transport / genetics RNA Interference Receptors, Notch Signal Transduction / genetics Signal Transduction / physiology* Sulfotransferases / genetics Sulfotransferases / isolation & purification Sulfotransferases / metabolism* |
| IF | 8.811 |
| 引用数 | 56 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | |