| RRC ID |
33692
|
| 著者 |
Hu CW, Tseng CW, Chien CW, Huang HC, Ku WC, Lee SJ, Chen YJ, Juan HF.
|
| タイトル |
Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development.
|
| ジャーナル |
J Proteome Res
|
| Abstract |
MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a.
|
| 巻・号 |
12(9)
|
| ページ |
3993-4004
|
| 公開日 |
2013-9-6
|
| DOI |
10.1021/pr400302w
|
| PMID |
23869555
|
| MeSH |
Alzheimer Disease / genetics
Alzheimer Disease / metabolism
Animals
Base Sequence
Brain / embryology
Brain / metabolism
Case-Control Studies
Cell Line, Tumor
Disease Progression
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs / physiology*
Parkinson Disease / genetics
Parkinson Disease / metabolism
Proteomics
RNA Interference
Sequence Homology, Nucleic Acid
Spinal Cord / embryology
Spinal Cord / metabolism
Stomach Neoplasms
Transcriptome*
Zebrafish
|
| IF |
4.074
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(HuC:Kaede)/rw0130a |
