論文 - 詳細
| RRC ID | 33692 |
|---|---|
| 著者 | Hu CW, Tseng CW, Chien CW, Huang HC, Ku WC, Lee SJ, Chen YJ, Juan HF. |
| タイトル | Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development. |
| ジャーナル | J Proteome Res |
| Abstract |
MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a. |
| 巻・号 | 12(9) |
| ページ | 3993-4004 |
| 公開日 | 2013-9-6 |
| DOI | 10.1021/pr400302w |
| PMID | 23869555 |
| MeSH | Alzheimer Disease / genetics Alzheimer Disease / metabolism Animals Base Sequence Brain / embryology Brain / metabolism Case-Control Studies Cell Line, Tumor Disease Progression Gene Expression Regulation, Neoplastic Humans MicroRNAs / physiology* Parkinson Disease / genetics Parkinson Disease / metabolism Proteomics RNA Interference Sequence Homology, Nucleic Acid Spinal Cord / embryology Spinal Cord / metabolism Stomach Neoplasms Transcriptome* Zebrafish |
| IF | 4.074 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(HuC:Kaede)/rw0130a |