RRC ID 33692
Author Hu CW, Tseng CW, Chien CW, Huang HC, Ku WC, Lee SJ, Chen YJ, Juan HF.
Title Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development.
Journal J Proteome Res
Abstract MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a.
Volume 12(9)
Pages 3993-4004
Published 2013-9-6
DOI 10.1021/pr400302w
PMID 23869555
MeSH Alzheimer Disease / genetics Alzheimer Disease / metabolism Animals Base Sequence Brain / embryology Brain / metabolism Case-Control Studies Cell Line, Tumor Disease Progression Gene Expression Regulation, Neoplastic Humans MicroRNAs / physiology* Parkinson Disease / genetics Parkinson Disease / metabolism Proteomics RNA Interference Sequence Homology, Nucleic Acid Spinal Cord / embryology Spinal Cord / metabolism Stomach Neoplasms Transcriptome* Zebrafish
IF 3.78
Times Cited 11
Zebrafish Tg(HuC:Kaede)/rw0130a