論文 - 詳細
| RRC ID | 3518 |
|---|---|
| 著者 | Lu C, Mains PE. |
| タイトル | The C. elegans anaphase promoting complex and MBK-2/DYRK kinase act redundantly with CUL-3/MEL-26 ubiquitin ligase to degrade MEI-1 microtubule-severing activity after meiosis. |
| ジャーナル | Dev Biol |
| Abstract |
The C. elegans embryo supports both meiotic and mitotic spindles, requiring careful regulation of components specific to each spindle type. The MEI-1/katanin microtubule-severing complex is required for meiosis but must be inactivated prior to mitosis. Downregulation of MEI-1 depends on MEL-26, which binds MEI-1, targeting it for degradation by the CUL-3 E3 ubiquitin ligase complex. Here we report that other protein degradation pathways, involving the anaphase promoting complex (APC) and the MBK-2/DYRK kinase, act in parallel to MEL-26 to inactivate MEI-1. At 25 degrees all mel-26(null) embryos die due to persistence of MEI-1 into mitosis, but at 15 degrees a significant portion of embryos hatch due to lower levels of ectopic MEI-1, suggesting that a redundant pathway also regulates MEI-1 degradation at 15 degrees. Previously the MBK-2/DYRK kinase was suggested to trigger MEL-26 mediated MEI-1 degradation. However, mbk-2 enhances the incomplete lethality of mel-26(null) at 15 degrees, arguing that MEL-26 acts in parallel to MBK-2. APC mutants behave similarly. In mel-26 embryos, ectopic MEI-1 remains until the onset of gastrulation, but in mbk-2; apc embryos, MEI-1 only persists through the first mitosis. We propose that mbk-2 and apc couple the initial phase of MEI-1 degradation to meiotic exit, after which MEL-26 completes MEI-1 degradation. |
| 巻・号 | 302(2) |
| ページ | 438-47 |
| 公開日 | 2007-2-15 |
| DOI | 10.1016/j.ydbio.2006.09.053 |
| PII | S0012-1606(06)01269-3 |
| PMID | 17069791 |
| MeSH | Adaptor Proteins, Signal Transducing / physiology* Adenosine Triphosphatases / metabolism* Anaphase-Promoting Complex-Cyclosome Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology* Dyrk Kinases Meiosis / physiology* Microtubules / metabolism Mitosis Mutation Protein Serine-Threonine Kinases / physiology* Protein-Tyrosine Kinases / physiology* Ubiquitin-Protein Ligase Complexes / genetics Ubiquitin-Protein Ligase Complexes / physiology* Ubiquitin-Protein Ligases / physiology* |
| IF | 2.896 |
| 引用数 | 26 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1664 |