論文 - 詳細
| RRC ID | 39772 |
|---|---|
| 著者 | Suizu F, Ueda K, Iwasaki T, Murata-Hori M, Hosoya H. |
| タイトル | Activation of actin-activated MgATPase activity of myosin II by phosphorylation with MAPK-activated protein kinase-1b (RSK-2). |
| ジャーナル | J Biochem |
| Abstract |
Regulatory light chain of myosin II (MRLC) was identified as a novel substrate of p90 ribosomal S6 kinase (RSK)-2, a Ser/Thr protein kinase which is phosphorylated and activated by mitogen-activated protein kinase (MAPK) in vitro and in vivo. Phosphopeptide map of MRLC phosphorylated by RSK-2 was identical to that by myosin light chain kinase (MLCK). Phosphoserine was recovered by the phosphoamino acid analysis of MRLC phosphorylated by RSK-2. Further, phosphorylation using recombinant glutathione S-transferase (GST) fusion proteins of HeLa MRLC2 revealed that RSK-2 phosphorylated wild-type MRLC2 (GST-wtMRLC2) but not its mutants GST-MRLC2(S19A) or GST-MRLC2(T18AS19A) (alanine substituted for Ser19 or both Ser19 and Thr18). These results revealed that RSK-2 phosphorylates MRLC at Ser19 as did MLCK. Phosphorylation of myosin II by RSK-2 resulted in activation of actin-activated MgATPase activity of myosin II. Interestingly, RSK-2 activity to phosphorylate MRLC was suppressed by phosphorylation with MAPK. RSK-2 might be a mediator that regulates myosin II activity through the MAPK cascade. |
| 巻・号 | 128(3) |
| ページ | 435-40 |
| 公開日 | 2000-9-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a022771 |
| PMID | 10965042 |
| MeSH | Actins / metabolism* Animals Blotting, Western Ca(2+) Mg(2+)-ATPase / metabolism* Cells, Cultured Chickens Electrophoresis, Polyacrylamide Gel Enzyme Activation Gizzard, Avian Glutathione Transferase / biosynthesis Humans MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases / metabolism Models, Molecular Muscle, Smooth / enzymology Myosins / metabolism* Peptide Fragments / isolation & purification Peptide Mapping Phosphopeptides / isolation & purification Phosphorylation Plasmids Precipitin Tests Protein Serine-Threonine Kinases / metabolism Recombinant Fusion Proteins / metabolism Ribosomal Protein S6 Kinases / metabolism* |
| IF | 2.476 |
| 引用数 | 23 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |