Reference - Detail
| RRC ID | 4182 |
|---|---|
| Author | Oishi K, Okano H, Sawa H. |
| Title | RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans. |
| Journal | J Cell Biol |
| Abstract |
For proper chromosome segregation, the sister kinetochores must attach to microtubules extending from the opposite spindle poles. Any errors in microtubule attachment can induce aneuploidy. In this study, we identify a novel conserved Caenorhabditis elegans microtubule-associated protein, regulator of microtubule dynamics 1 (RMD-1), that localizes to spindle microtubules and spindle poles. Depletion of RMD-1 induces severe defects in chromosome segregation, probably through merotelic attachments between microtubules and chromosomes. Although rmd-1 embryos also have a mild defect in microtubule growth, we find that mutants of the microtubule growth regulator XMAP215/ZYG-9 show much weaker segregation defects. This suggests that the microtubule growth defect in rmd-1 embryos does not cause abnormal chromosome segregation. We also see that RMD-1 interacts with aurora B in vitro. Our results suggest that RMD-1 functions in chromosome segregation in C. elegans embryos, possibly through the aurora B-mediated pathway. Human homologues of RMD-1 could also bind microtubules, which would suggest a function for these proteins in chromosome segregation during mitosis in other organisms as well. |
| Volume | 179(6) |
| Pages | 1149-62 |
| Published | 2007-12-17 |
| DOI | 10.1083/jcb.200705108 |
| PII | jcb.200705108 |
| PMID | 18070910 |
| PMC | PMC2140014 |
| MeSH | Amino Acid Sequence Animals Aurora Kinase B Aurora Kinases Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Chromosome Segregation / physiology* Embryo, Nonmammalian / metabolism Embryo, Nonmammalian / ultrastructure Humans Kinetochores / metabolism Mice Microtubule-Associated Proteins / chemistry Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / physiology* Microtubules / metabolism Microtubules / ultrastructure Molecular Sequence Data Protein Serine-Threonine Kinases / metabolism Protein Structure, Tertiary Sequence Alignment Spindle Apparatus / metabolism Spindle Apparatus / ultrastructure Xenopus laevis Zebrafish / metabolism |
| IF | 8.811 |
| Times Cited | 17 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| C.elegans | tm1457 |