Reference - Detail
| RRC ID | 42770 |
|---|---|
| Author | Shinozaki-Narikawa N, Kodama T, Shibasaki Y. |
| Title | Cooperation of phosphoinositides and BAR domain proteins in endosomal tubulation. |
| Journal | Traffic |
| Abstract |
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate many intracellular events, including vesicular trafficking and actin remodeling, by recruiting proteins to their sites of function. PtdIns(4,5)-bisphosphate [PI(4,5)P2] and related phosphoinositides are mainly synthesized by type I PtdIns-4-phosphate 5-kinases (PIP5Ks). We found that PIP5K induces endosomal tubules in COS-7 cells. ADP-ribosylation factor (ARF) 6 has been shown to act upstream of PIP5K and regulate endocytic transport and tubulation. ARF GAP with coiled-coil, ankyrin repeat, and pleckstrin homology domains 1 (ACAP1) has guanosine triphosphatase-activating protein (GAP) activity for ARF6. While there were few tubules induced by the expression of ACAP1 alone, numerous endosomal tubules were induced by coexpression of PIP5K and ACAP1. ACAP1 has a pleckstrin homology (PH) domain known to bind phosphoinositide and a Bin/amphiphysin/Rvs (BAR) domain that has been reported to detect membrane curvature. Truncated and point mutations in the ACAP1 BAR and PH domains revealed that both BAR and PH domains are required for tubulation. These results suggest that two ARF6 downstream molecules, PIP5K and ACAP1, function together in endosomal tubulation and that phosphoinositide levels may regulate endosomal dynamics. |
| Volume | 7(11) |
| Pages | 1539-50 |
| Published | 2006-11-1 |
| DOI | 10.1111/j.1600-0854.2006.00480.x |
| PII | TRA480 |
| PMID | 17010122 |
| MeSH | ADP-Ribosylation Factor 6 Animals Binding Sites / genetics COS Cells Chlorocebus aethiops Endosomes / drug effects Endosomes / metabolism* GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism* Humans Isoenzymes / genetics Isoenzymes / metabolism Mice Microtubules / drug effects Microtubules / metabolism Mutation / genetics Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nocodazole / pharmacology Phosphatidylinositol 4,5-Diphosphate / metabolism Phosphatidylinositols / metabolism* Phospholipase C delta Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism* Protein Binding Recombinant Fusion Proteins / metabolism Transfection Tubulin / metabolism Type C Phospholipases / genetics Type C Phospholipases / metabolism |
| IF | 4.045 |
| Times Cited | 28 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |