論文 - 詳細
| RRC ID | 45687 |
|---|---|
| 著者 | Barberán-Soler S, Fontrodona L, Ribó A, Lamm AT, Iannone C, Cerón J, Lehner B, Valcárcel J. |
| タイトル | Co-option of the piRNA pathway for germline-specific alternative splicing of C. elegans TOR. |
| ジャーナル | Cell Rep |
| Abstract |
Many eukaryotic genes contain embedded antisense transcripts and repetitive sequences of unknown function. We report that male germline-specific expression of an antisense transcript contained in an intron of C. elegans Target of Rapamycin (TOR, let-363) is associated with (1) accumulation of endo-small interfering RNAs (siRNAs) against an embedded Helitron transposon and (2) activation of an alternative 3' splice site of TOR. The germline-specific Argonaute proteins PRG-1 and CSR-1, which participate in self/nonself RNA recognition, antagonistically regulate the generation of these endo-siRNAs, TOR mRNA levels, and 3' splice-site selection. Supply of exogenous double-stranded RNA against the region of sense/antisense overlap reverses changes in TOR expression and splicing and suppresses the progressive multigenerational sterility phenotype of prg-1 mutants. We propose that recognition of a "nonself" intronic transposon by endo-siRNAs/the piRNA system provides physiological regulation of expression and alternative splicing of a host gene that, in turn, contributes to the maintenance of germline function across generations. |
| 巻・号 | 8(6) |
| ページ | 1609-1616 |
| 公開日 | 2014-9-25 |
| DOI | 10.1016/j.celrep.2014.08.016 |
| PII | S2211-1247(14)00679-2 |
| PMID | 25220461 |
| MeSH | Alternative Splicing Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Germ Cells Male Mutation Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference RNA, Antisense / metabolism RNA, Double-Stranded / metabolism RNA, Small Interfering / metabolism* Sequence Analysis, RNA TOR Serine-Threonine Kinases / genetics* TOR Serine-Threonine Kinases / metabolism |
| IF | 8.109 |
| 引用数 | 12 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 tm892 |