| RRC ID |
45687
|
| Author |
Barberán-Soler S, Fontrodona L, Ribó A, Lamm AT, Iannone C, Cerón J, Lehner B, Valcárcel J.
|
| Title |
Co-option of the piRNA pathway for germline-specific alternative splicing of C. elegans TOR.
|
| Journal |
Cell Rep
|
| Abstract |
Many eukaryotic genes contain embedded antisense transcripts and repetitive sequences of unknown function. We report that male germline-specific expression of an antisense transcript contained in an intron of C. elegans Target of Rapamycin (TOR, let-363) is associated with (1) accumulation of endo-small interfering RNAs (siRNAs) against an embedded Helitron transposon and (2) activation of an alternative 3' splice site of TOR. The germline-specific Argonaute proteins PRG-1 and CSR-1, which participate in self/nonself RNA recognition, antagonistically regulate the generation of these endo-siRNAs, TOR mRNA levels, and 3' splice-site selection. Supply of exogenous double-stranded RNA against the region of sense/antisense overlap reverses changes in TOR expression and splicing and suppresses the progressive multigenerational sterility phenotype of prg-1 mutants. We propose that recognition of a "nonself" intronic transposon by endo-siRNAs/the piRNA system provides physiological regulation of expression and alternative splicing of a host gene that, in turn, contributes to the maintenance of germline function across generations.
|
| Volume |
8(6)
|
| Pages |
1609-1616
|
| Published |
2014-9-25
|
| DOI |
10.1016/j.celrep.2014.08.016
|
| PII |
S2211-1247(14)00679-2
|
| PMID |
25220461
|
| MeSH |
Alternative Splicing
Animals
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Germ Cells
Male
Mutation
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Interference
RNA, Antisense / metabolism
RNA, Double-Stranded / metabolism
RNA, Small Interfering / metabolism*
Sequence Analysis, RNA
TOR Serine-Threonine Kinases / genetics*
TOR Serine-Threonine Kinases / metabolism
|
| IF |
8.109
|
| Times Cited |
12
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm872
tm892 |
