RRC ID 45687
著者 Barberán-Soler S, Fontrodona L, Ribó A, Lamm AT, Iannone C, Cerón J, Lehner B, Valcárcel J.
タイトル Co-option of the piRNA pathway for germline-specific alternative splicing of C. elegans TOR.
ジャーナル Cell Rep
Abstract Many eukaryotic genes contain embedded antisense transcripts and repetitive sequences of unknown function. We report that male germline-specific expression of an antisense transcript contained in an intron of C. elegans Target of Rapamycin (TOR, let-363) is associated with (1) accumulation of endo-small interfering RNAs (siRNAs) against an embedded Helitron transposon and (2) activation of an alternative 3' splice site of TOR. The germline-specific Argonaute proteins PRG-1 and CSR-1, which participate in self/nonself RNA recognition, antagonistically regulate the generation of these endo-siRNAs, TOR mRNA levels, and 3' splice-site selection. Supply of exogenous double-stranded RNA against the region of sense/antisense overlap reverses changes in TOR expression and splicing and suppresses the progressive multigenerational sterility phenotype of prg-1 mutants. We propose that recognition of a "nonself" intronic transposon by endo-siRNAs/the piRNA system provides physiological regulation of expression and alternative splicing of a host gene that, in turn, contributes to the maintenance of germline function across generations.
巻・号 8(6)
ページ 1609-1616
公開日 2014-9-25
DOI 10.1016/j.celrep.2014.08.016
PII S2211-1247(14)00679-2
PMID 25220461
MeSH Alternative Splicing Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Germ Cells Male Mutation Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference RNA, Antisense / metabolism RNA, Double-Stranded / metabolism RNA, Small Interfering / metabolism* Sequence Analysis, RNA TOR Serine-Threonine Kinases / genetics* TOR Serine-Threonine Kinases / metabolism
IF 8.109
引用数 12
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm872 tm892