| RRC ID |
45885
|
| Author |
Wolters S, Ermolaeva MA, Bickel JS, Fingerhut JM, Khanikar J, Chan RC, Schumacher B.
|
| Title |
Loss of Caenorhabditis elegans BRCA1 promotes genome stability during replication in smc-5 mutants.
|
| Journal |
Genetics
|
| Abstract |
DNA damage by ultraviolet (UV) light poses a risk for mutagenesis and a potential hindrance for cell cycle progression. Cells cope with UV-induced DNA damage through two general strategies to repair the damaged nucleotides and to promote cell cycle progression in the presence of UV-damaged DNA. Defining the genetic pathways and understanding how they function together to enable effective tolerance to UV remains an important area of research. The structural maintenance of chromosomes (SMC) proteins form distinct complexes that maintain genome stability during chromosome segregation, homologous recombination, and DNA replication. Using a forward genetic screen, we identified two alleles of smc-5 that exacerbate UV sensitivity in Caenorhabditis elegans. Germ cells of smc-5-defective animals show reduced proliferation, sensitivity to perturbed replication, chromatin bridge formation, and accumulation of RAD-51 foci that indicate the activation of homologous recombination at DNA double-strand breaks. Mutations in the translesion synthesis polymerase polh-1 act synergistically with smc-5 mutations in provoking genome instability after UV-induced DNA damage. In contrast, the DNA damage accumulation and sensitivity of smc-5 mutant strains to replication impediments are suppressed by mutations in the C. elegans BRCA1/BARD1 homologs, brc-1 and brd-1. We propose that SMC-5/6 promotes replication fork stability and facilitates recombination-dependent repair when the BRC-1/BRD-1 complex initiates homologous recombination at stalled replication forks. Our data suggest that BRC-1/BRD-1 can both promote and antagonize genome stability depending on whether homologous recombination is initiated during DNA double-strand break repair or during replication stalling.
|
| Volume |
196(4)
|
| Pages |
985-99
|
| Published |
2014-4-1
|
| DOI |
10.1534/genetics.113.158295
|
| PII |
genetics.113.158295
|
| PMID |
24424777
|
| PMC |
PMC3982690
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism*
Cell Cycle Proteins / genetics*
Cell Cycle Proteins / metabolism
DNA Damage
DNA Replication / radiation effects
DNA, Helminth
Genome, Helminth
Genomic Instability* / radiation effects
Germ Cells / metabolism
Mutation
Rad51 Recombinase / metabolism
Tumor Suppressor Proteins / genetics
Tumor Suppressor Proteins / metabolism*
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
|
| IF |
4.015
|
| Times Cited |
19
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| C.elegans |
tm3886
tm1145 |
