| RRC ID |
45916
|
| Author |
Wu Y, Cheng S, Zhao H, Zou W, Yoshina S, Mitani S, Zhang H, Wang X.
|
| Title |
PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation.
|
| Journal |
EMBO Rep
|
| Abstract |
Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphatase MTM-3 catalyzes PtdIns3P turnover late in autophagy. MTM-3 acts downstream of the ATG-2/EPG-6 complex and upstream of EPG-5 to promote autophagosome maturation into autolysosomes. MTM-3 is recruited to autophagosomes by PtdIns3P, and loss of MTM-3 causes increased autophagic association of ATG-18 in a PtdIns3P-dependent manner. Our data reveal critical roles of PtdIns3P turnover in autophagosome maturation and/or autolysosome formation.
|
| Volume |
15(9)
|
| Pages |
973-81
|
| Published |
2014-9-1
|
| DOI |
10.15252/embr.201438618
|
| PII |
embr.201438618
|
| PMID |
25124690
|
| PMC |
PMC4198041
|
| MeSH |
Animals
Autophagy / genetics*
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism
Lysosomes / genetics
Lysosomes / metabolism
Phagosomes / genetics*
Phosphatidylinositol 3-Kinases / genetics
Phosphatidylinositol 3-Kinases / metabolism*
Phosphatidylinositol Phosphates / metabolism*
Phosphoric Monoester Hydrolases / metabolism
Protein Tyrosine Phosphatases, Non-Receptor / metabolism
|
| IF |
7.497
|
| Times Cited |
26
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm4475 |
