Reference - Detail
RRC ID | 46196 |
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Author | Shibata Y, Sawa H, Nishiwaki K. |
Title | HTZ-1/H2A.z and MYS-1/MYST HAT act redundantly to maintain cell fates in somatic gonadal cells through repression of ceh-22 in C. elegans. |
Journal | Development |
Abstract |
The stable maintenance of acquired cell fates is important during development and for maintaining tissue homeostasis. Although histone modification is one of the major strategies used by cells to maintain their fates, the mechanisms by which histone variants maintain cell fates are not well understood. In C. elegans, the acetylated-histone-H4 (AcH4)-binding protein BET-1 acts downstream of the MYST family histone acetyltransferases MYS-1 and MYS-2 to establish and maintain cell fates in multiple cell lineages. Here we show that, in the bet-1 pathway, the histone H2A variant HTZ-1/H2A.z and MYS-1 are required for the maintenance of cell fates in a redundant manner. BET-1 controlled the subnuclear localization of HTZ-1. HTZ-1 and MYS-1 maintained the fates of the somatic gonadal cells (SGCs) through the repression of a target, ceh-22/Nkx2.5, which induced the formation of the leader cells of the gonad. H3K27 demethylase, UTX-1, had an antagonistic effect relative to HTZ-1 in the regulation of ceh-22. Nuclear spot assay revealed that HTZ-1 localized to the ceh-22 locus in SGCs in an utx-1-dependent manner. We propose that HTZ-1 and MYS-1 repress ceh-22 when UTX-1 removes its silencing mark, H3K27 methylation on the ceh-22 locus, thereby maintaining the fates of SGCs. |
Volume | 141(1) |
Pages | 209-18 |
Published | 2014-1-1 |
DOI | 10.1242/dev.090746 |
PII | 141/1/209 |
PMID | 24346701 |
MeSH | Adenosine Triphosphatases / genetics Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Cell Lineage Chromosomal Proteins, Non-Histone / genetics DNA Helicases / genetics DNA Methylation Gene Expression Regulation, Developmental Gonads / cytology Gonads / embryology* Histone Acetyltransferases / metabolism* Histone Demethylases / genetics Histone Demethylases / metabolism* Histones / metabolism* Homeodomain Proteins / biosynthesis Homeodomain Proteins / metabolism* Nuclear Proteins / genetics Nuclear Proteins / metabolism RNA Interference RNA, Small Interfering / genetics Trans-Activators / genetics Transcription Factors / biosynthesis Transcription Factors / metabolism* |
IF | 5.611 |
Times Cited | 5 |
WOS Category | DEVELOPMENTAL BIOLOGY |
Resource | |
C.elegans | tm2469 |