論文 - 詳細
| RRC ID | 46251 |
|---|---|
| 著者 | Ding YH, Du YG, Luo S, Li YX, Li TM, Yoshina S, Wang X, Klage K, Mitani S, Ye K, Dong MQ. |
| タイトル | Characterization of PUD-1 and PUD-2, two proteins up-regulated in a long-lived daf-2 mutant. |
| ジャーナル | PLoS One |
| Abstract |
C. elegans PUD-1 and PUD-2, two proteins up-regulated in daf-2(loss-of-function) (PUD), are homologous 17-kD proteins with a large abundance increase in long-lived daf-2 mutant animals of reduced insulin signaling. In this study, we show that both PUD-1 and PUD-2 are abundantly expressed in the intestine and hypodermis, and form a heterodimer. We have solved their crystal structure to 1.9-Å resolution and found that both proteins adopt similar β-sandwich folds in the V-shaped dimer. In contrast, their homologs PUD-3, PUD-4, PUDL-1 and PUDL-2 are all monomeric proteins with distinct expression patterns in C. elegans. Thus, the PUD-1/PUD-2 heterodimer probably has a function distinct from their family members. Neither overexpression nor deletion of pud-1 and pud-2 affected the lifespan of WT or daf-2 mutant animals, suggesting that their induction in daf-2 worms does not contribute to longevity. Curiously, deletion of pud-1 and pud-2 was associated with a protective effect against paralysis induced by the amyloid β-peptide (1-42), which further enhanced the protection conferred by daf-2(RNAi) against Aβ. |
| 巻・号 | 8(6) |
| ページ | e67158 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0067158 |
| PII | PONE-D-13-17083 |
| PMID | 23799143 |
| PMC | PMC3683130 |
| MeSH | Amino Acid Sequence Amyloid beta-Peptides / pharmacology Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Crystallography, X-Ray Forkhead Transcription Factors Gene Expression Hydrophobic and Hydrophilic Interactions Longevity Models, Molecular Molecular Sequence Data Peptide Fragments / pharmacology Protein Multimerization Protein Structure, Quaternary Protein Structure, Secondary Protein Structure, Tertiary Receptor, Insulin / genetics* Sequence Homology, Amino Acid Stress, Physiological Transcription Factors / metabolism Up-Regulation |
| IF | 2.74 |
| 引用数 | 8 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6107 tm5053 |