| RRC ID |
46251
|
| 著者 |
Ding YH, Du YG, Luo S, Li YX, Li TM, Yoshina S, Wang X, Klage K, Mitani S, Ye K, Dong MQ.
|
| タイトル |
Characterization of PUD-1 and PUD-2, two proteins up-regulated in a long-lived daf-2 mutant.
|
| ジャーナル |
PLoS One
|
| Abstract |
C. elegans PUD-1 and PUD-2, two proteins up-regulated in daf-2(loss-of-function) (PUD), are homologous 17-kD proteins with a large abundance increase in long-lived daf-2 mutant animals of reduced insulin signaling. In this study, we show that both PUD-1 and PUD-2 are abundantly expressed in the intestine and hypodermis, and form a heterodimer. We have solved their crystal structure to 1.9-Å resolution and found that both proteins adopt similar β-sandwich folds in the V-shaped dimer. In contrast, their homologs PUD-3, PUD-4, PUDL-1 and PUDL-2 are all monomeric proteins with distinct expression patterns in C. elegans. Thus, the PUD-1/PUD-2 heterodimer probably has a function distinct from their family members. Neither overexpression nor deletion of pud-1 and pud-2 affected the lifespan of WT or daf-2 mutant animals, suggesting that their induction in daf-2 worms does not contribute to longevity. Curiously, deletion of pud-1 and pud-2 was associated with a protective effect against paralysis induced by the amyloid β-peptide (1-42), which further enhanced the protection conferred by daf-2(RNAi) against Aβ.
|
| 巻・号 |
8(6)
|
| ページ |
e67158
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0067158
|
| PII |
PONE-D-13-17083
|
| PMID |
23799143
|
| PMC |
PMC3683130
|
| MeSH |
Amino Acid Sequence
Amyloid beta-Peptides / pharmacology
Animals
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Crystallography, X-Ray
Forkhead Transcription Factors
Gene Expression
Hydrophobic and Hydrophilic Interactions
Longevity
Models, Molecular
Molecular Sequence Data
Peptide Fragments / pharmacology
Protein Multimerization
Protein Structure, Quaternary
Protein Structure, Secondary
Protein Structure, Tertiary
Receptor, Insulin / genetics*
Sequence Homology, Amino Acid
Stress, Physiological
Transcription Factors / metabolism
Up-Regulation
|
| IF |
2.74
|
| 引用数 |
8
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm6107
tm5053 |
