RRC ID 46717
Author Chen D, Xiao H, Zhang K, Wang B, Gao Z, Jian Y, Qi X, Sun J, Miao L, Yang C.
Title Retromer is required for apoptotic cell clearance by phagocytic receptor recycling.
Journal Science
Abstract The cell surface receptor CED-1 mediates apoptotic cell recognition by phagocytic cells, enabling cell corpse clearance in Caenorhabditis elegans. Here, we found that the C. elegans intracellular protein sorting complex, retromer, was required for cell corpse clearance by mediating the recycling of CED-1. Retromer was recruited to the surfaces of phagosomes containing cell corpses, and its loss of function caused defective cell corpse removal. The retromer probably acted through direct interaction with CED-1 in the cell corpse recognition pathway. In the absence of retromer function, CED-1 associated with lysosomes and failed to recycle from phagosomes and cytosol to the plasma membrane. Thus, retromer is an essential mediator of apoptotic cell clearance by regulating phagocytic receptor(s) during cell corpse engulfment.
Volume 327(5970)
Pages 1261-4
Published 2010-3-5
DOI 10.1126/science.1184840
PII science.1184840
PMID 20133524
MeSH Amino Acid Sequence Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism Lysosomes / metabolism Membrane Proteins / metabolism* Microscopy, Electron, Transmission Molecular Sequence Data Phagocytosis* Phagosomes / metabolism* Protein Transport* RNA Interference Recombinant Fusion Proteins / metabolism Sorting Nexins Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism*
IF 41.846
Times Cited 83
C.elegans tm847 tm3790 tm1320 tm2423 tm1523