| RRC ID |
46744
|
| 著者 |
Ward JD, Muzzini DM, Petalcorin MI, Martinez-Perez E, Martin JS, Plevani P, Cassata G, Marini F, Boulton SJ.
|
| タイトル |
Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair.
|
| ジャーナル |
Mol Cell
|
| Abstract |
Homologous recombination (HR) is essential for repair of meiotic DNA double-strand breaks (DSBs). Although the mechanisms of RAD-51-DNA filament assembly and strand exchange are well characterized, the subsequent steps of HR are less well defined. Here, we describe a synthetic lethal interaction between the C. elegans helicase helq-1 and RAD-51 paralog rfs-1, which results in a block to meiotic DSB repair after strand invasion. Whereas RAD-51-ssDNA filaments assemble at meiotic DSBs with normal kinetics in helq-1, rfs-1 double mutants, persistence of RAD-51 foci and genetic interactions with rtel-1 suggest a failure to disassemble RAD-51 from strand invasion intermediates. Indeed, purified HELQ-1 and RFS-1 independently bind to and promote the disassembly of RAD-51 from double-stranded, but not single-stranded, DNA filaments via distinct mechanisms in vitro. These results indicate that two compensating activities are required to promote postsynaptic RAD-51 filament disassembly, which are collectively essential for completion of meiotic DSB repair.
|
| 巻・号 |
37(2)
|
| ページ |
259-72
|
| 公開日 |
2010-1-29
|
| DOI |
10.1016/j.molcel.2009.12.026
|
| PII |
S1097-2765(09)00957-5
|
| PMID |
20122407
|
| MeSH |
Animals
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Caenorhabditis elegans Proteins / physiology*
DNA Breaks, Double-Stranded*
DNA Helicases / genetics
DNA Helicases / metabolism
DNA Helicases / physiology
DNA Repair / genetics
DNA Repair / physiology*
DNA, Helminth / metabolism
DNA, Single-Stranded / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
DNA-Binding Proteins / physiology*
Meiosis*
Mutation
Rad51 Recombinase / metabolism*
Recombination, Genetic
|
| IF |
15.584
|
| 引用数 |
54
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2134
tm1866
tm1086
tm1268 |
