RRC ID 49508
著者 Kondo Y, Hanai A, Nakai W, Katoh Y, Nakayama K, Shin HW.
タイトル ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway.
ジャーナル Cell Struct Funct
Abstract Small GTPases ARF1 and ARF3 localize mainly to the Golgi apparatus, where they trigger formation of coated carrier vesicles. We previously showed that BIG2, a guanine nucleotide exchange factor specific for ARF1 and ARF3, localizes not only to the trans-Golgi network (TGN) but also to recycling endosomes, where it is involved in regulating the integrity of recycling endosomes. However, it is not yet clear whether ARF1 and ARF3 act downstream of BIG2 to ensure endosome integrity. In this study, we show that EGFP-tagged ARF1 and ARF3 localize to endosomal compartments containing endocytosed transferrin. We further demonstrate that simultaneous depletion of ARF1 and ARF3 induces tubulation of recycling endosomal compartments positive for transferrin receptor, Rab4, and Rab11, but does not significantly affect the integrity of the Golgi apparatus or early or late endosomes. Moreover, the simultaneous depletion of ARF1 and ARF3 suppresses recycling of transferrin but does not affect either its endocytosis or the retrograde transport of TGN38 from early/recycling endosomes to the TGN. In addition, depletion of ARF1 and ARF3 does not affect retrograde transport of CD4-furin from late endosomes to the TGN, or of endocytosed EGF from late endosomes to lysosomes. These results indicate that ARF1 and ARF3 are redundantly required for the integrity of recycling endosomes, and that they regulate transferrin recycling from endosomes to the plasma membrane, but not retrograde transport from endosomal compartments to the TGN.
巻・号 37(2)
ページ 141-54
公開日 2012-1-1
DOI 10.1247/csf.12015
PII DN/JST.JSTAGE/csf/12015
PMID 22971977
MeSH ADP-Ribosylation Factor 1 / deficiency ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* ADP-Ribosylation Factors / deficiency ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism* Cell Membrane / metabolism Endosomes / metabolism* Gene Knockdown Techniques Golgi Apparatus / metabolism HeLa Cells Humans Lysosomes / metabolism Microtubules / metabolism Protein Transport Receptors, Transferrin / metabolism Transferrin / metabolism rab GTP-Binding Proteins / metabolism rab4 GTP-Binding Proteins / metabolism
IF 2.429
引用数 22
リソース情報
遺伝子材料 pcDNA3/hArf1(WT)-HA (RDB15337) pcDNA3/mArf1(Q71L)-HA (RDB15338) pcDNA3/mArf1(T31N)-HA (RDB15339) pEGFP-N3/hArf1(WT)-EGFP (RDB15340) pcDNA3/hArf3(WT)-HA (RDB15341) pcDNA3/mArf3(Q71L)-HA (RDB15342) pcDNA3/mArf3(T31N)-HA (RDB15343) pEGFP-N3/hArf3-EGFP (RDB15344).