| RRC ID |
49512
|
| 著者 |
Ishizaki R, Shin HW, Mitsuhashi H, Nakayama K.
|
| タイトル |
Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes.
|
| ジャーナル |
Mol Biol Cell
|
| Abstract |
BIG2 and BIG1 are closely related guanine-nucleotide exchange factors (GEFs) for ADP-ribosylation factors (ARFs) and are involved in the regulation of membrane traffic through activating ARFs and recruiting coat protein complexes, such as the COPI complex and the AP-1 clathrin adaptor complex. Although both ARF-GEFs are associated mainly with the trans-Golgi network (TGN) and BIG2 is also associated with recycling endosomes, it is unclear whether BIG2 and BIG1 share some roles in membrane traffic. We here show that knockdown of both BIG2 and BIG1 by RNAi causes mislocalization of a subset of proteins associated with the TGN and recycling endosomes and blocks retrograde transport of furin from late endosomes to the TGN. Similar mislocalization and protein transport block, including furin, were observed in cells depleted of AP-1. Taken together with previous reports, these observations indicate that BIG2 and BIG1 play redundant roles in trafficking between the TGN and endosomes that involves the AP-1 complex.
|
| 巻・号 |
19(6)
|
| ページ |
2650-60
|
| 公開日 |
2008-6-1
|
| DOI |
10.1091/mbc.e07-10-1067
|
| PII |
E07-10-1067
|
| PMID |
18417613
|
| PMC |
PMC2397321
|
| MeSH |
ADP-Ribosylation Factors / metabolism*
Adaptor Protein Complex 1 / metabolism
Amino Acid Sequence
CD4 Antigens / metabolism
Cell Membrane / metabolism
Endosomes / metabolism*
Furin / chemistry
Furin / metabolism
Guanine Nucleotide Exchange Factors / metabolism*
HeLa Cells
Humans
Intracellular Membranes / metabolism*
Models, Biological
Molecular Sequence Data
Mutation / genetics
Phenotype
Protein Transport
Receptors, Transferrin / metabolism
trans-Golgi Network / metabolism*
|
| IF |
3.791
|
| 引用数 |
59
|
| リソース情報 |
| 遺伝子材料 |
pcDNA4c/His-Xpress-HA-hBIG2(WT) (RDB15364)
pcDNA4c/His-Xpress-HA-hBIG2(E738K) (RDB15365). |
