RRC ID 53246
Author Neal SJ, Park J, DiTirro D, Yoon J, Shibuya M, Choi W, Schroeder FC, Butcher RA, Kim K, Sengupta P.
Title A Forward Genetic Screen for Molecules Involved in Pheromone-Induced Dauer Formation in Caenorhabditis elegans.
Journal G3 (Bethesda)
Abstract Animals must constantly assess their surroundings and integrate sensory cues to make appropriate behavioral and developmental decisions. Pheromones produced by conspecific individuals provide critical information regarding environmental conditions. Ascaroside pheromone concentration and composition are instructive in the decision of Caenorhabditis elegans to either develop into a reproductive adult or enter into the stress-resistant alternate dauer developmental stage. Pheromones are sensed by a small set of sensory neurons, and integrated with additional environmental cues, to regulate neuroendocrine signaling and dauer formation. To identify molecules required for pheromone-induced dauer formation, we performed an unbiased forward genetic screen and identified phd (pheromone response-defective dauer) mutants. Here, we describe new roles in dauer formation for previously identified neuronal molecules such as the WD40 domain protein QUI-1 and MACO-1 Macoilin, report new roles for nociceptive neurons in modulating pheromone-induced dauer formation, and identify tau tubulin kinases as new genes involved in dauer formation. Thus, phd mutants define loci required for the detection, transmission, or integration of pheromone signals in the regulation of dauer formation.
Volume 6(5)
Pages 1475-87
Published 2016-5-3
DOI 10.1534/g3.115.026450
PII g3.115.026450
PMID 26976437
PMC PMC4856098
MeSH Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Chromosome Mapping Genetic Association Studies* Genetic Complementation Test Genetic Linkage Genetic Testing* Genome, Helminth Genomics / methods High-Throughput Nucleotide Sequencing Mutation Pheromones / pharmacology* Signal Transduction / drug effects Stress, Physiological / drug effects* Stress, Physiological / genetics*
IF 2.781
Times Cited 8
C.elegans tm4006 tm4134 tm3841 tm4852 tm1946 tm2943