RRC ID 53956
Author Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P, Snijders AP, Colaiácovo MP, Martinez-Perez E.
Title Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis.
Journal Nat Commun
Abstract The formation of haploid gametes from diploid germ cells requires the regulated two-step release of sister chromatid cohesion (SCC) during the meiotic divisions. Here, we show that phosphorylation of cohesin subunit REC-8 by Aurora B promotes SCC release at anaphase I onset in C. elegans oocytes. Aurora B loading to chromatin displaying Haspin-mediated H3 T3 phosphorylation induces spatially restricted REC-8 phosphorylation, preventing full SCC release during anaphase I. H3 T3 phosphorylation is locally antagonized by protein phosphatase 1, which is recruited to chromosomes by HTP-1/2 and LAB-1. Mutating the N terminus of HTP-1 causes ectopic H3 T3 phosphorylation, triggering precocious SCC release without impairing earlier HTP-1 roles in homolog pairing and recombination. CDK-1 exerts temporal regulation of Aurora B recruitment, coupling REC-8 phosphorylation to oocyte maturation. Our findings elucidate a complex regulatory network that uses chromosome axis components, H3 T3 phosphorylation, and cell cycle regulators to ensure accurate chromosome segregation during oogenesis.
Volume 9(1)
Pages 834
Published 2018-2-26
DOI 10.1038/s41467-018-03229-5
PII 10.1038/s41467-018-03229-5
PMID 29483514
PMC PMC5827026
MeSH Anaphase Animals Aurora Kinase B / genetics* Aurora Kinase B / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Chromatids / metabolism Chromatids / ultrastructure Chromatin / metabolism Chromatin / ultrastructure Chromosomal Proteins, Non-Histone / genetics* Chromosomal Proteins, Non-Histone / metabolism Chromosome Segregation Gene Expression Regulation Hermaphroditic Organisms / cytology Hermaphroditic Organisms / genetics* Hermaphroditic Organisms / metabolism Histones / genetics Histones / metabolism Oocytes / cytology Oocytes / metabolism* Oogenesis / genetics Phosphorylation Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism
IF 11.878
Times Cited 13
Resource
C.elegans tm1791 tm1395 tm2543