論文 - 詳細
| RRC ID | 54448 |
|---|---|
| 著者 | Tay YD, Leda M, Spanos C, Rappsilber J, Goryachev AB, Sawin KE. |
| タイトル | Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex. |
| ジャーナル | Cell Rep |
| Abstract |
NDR/LATS kinases regulate multiple aspects of cell polarity and morphogenesis from yeast to mammals. Fission yeast NDR/LATS kinase Orb6 has been proposed to control cell polarity by regulating the Cdc42 guanine nucleotide exchange factor Gef1. Here, we show that Orb6 regulates polarity largely independently of Gef1 and that Orb6 positively regulates exocytosis. Through Orb6 inhibition in vivo and quantitative global phosphoproteomics, we identify Orb6 targets, including proteins involved in membrane trafficking. We confirm Sec3 and Sec5, conserved components of the exocyst complex, as substrates of Orb6 both in vivo and in vitro, and we show that Orb6 kinase activity is important for exocyst localization to cell tips and for exocyst activity during septum dissolution after cytokinesis. We further find that Orb6 phosphorylation of Sec3 contributes to exocyst function in concert with exocyst protein Exo70. We propose that Orb6 contributes to polarized growth by regulating membrane trafficking at multiple levels. |
| 巻・号 | 26(6) |
| ページ | 1654-1667.e7 |
| 公開日 | 2019-2-5 |
| DOI | 10.1016/j.celrep.2019.01.027 |
| PII | S2211-1247(19)30036-1 |
| PMID | 30726745 |
| PMC | PMC6367570 |
| MeSH | Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cell Polarity Cytokinesis / genetics Exocytosis / genetics* Gene Expression Regulation, Fungal* Phosphoproteins / classification Phosphoproteins / genetics* Phosphoproteins / metabolism Phosphorylation Protein Isoforms / genetics Protein Isoforms / metabolism Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism Proteomics / methods Rho Guanine Nucleotide Exchange Factors / genetics Rho Guanine Nucleotide Exchange Factors / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Vesicular Transport Proteins / genetics* Vesicular Transport Proteins / metabolism |
| IF | 7.815 |
| 引用数 | 4 |
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