RRC ID 5651
Author Higuchi N, Kohno K, Kadowaki T.
Title Specific retention of the protostome-specific PsGEF may parallel with the evolution of mushroom bodies in insect and lophotrochozoan brains.
Journal BMC Biol.
Abstract BACKGROUND:Gene gain and subsequent retention or loss during evolution may be one of the underlying mechanisms involved in generating the diversity of metazoan nervous systems. However, the causal relationships acting therein have not been studied extensively.
RESULTS:We identified the gene PsGEF (protostome-specific GEF), which is present in all the sequenced genomes of insects and limpet but absent in those of sea anemones, deuterostomes, and nematodes. In Drosophila melanogaster, PsGEF encodes a short version of a protein with the C2 and PDZ domains, as well as a long version with the C2, PDZ, and RhoGEF domains through alternative splicing. Intriguingly, the exons encoding the RhoGEF domain are specifically deleted in the Daphnia pulex genome, suggesting that Daphnia PsGEF contains only the C2 and PDZ domains. Thus, the distribution of PsGEF containing the C2, PDZ, and RhoGEF domains among metazoans appears to coincide with the presence of mushroom bodies. Mushroom bodies are prominent neuropils involved in the processing of multiple sensory inputs as well as associative learning in the insect, platyhelminth, and annelid brains. In the adult Drosophila brain, PsGEF is expressed in mushroom bodies, antennal lobe, and optic lobe, where it is necessary for the correct axon branch formation of alpha/beta neurons in mushroom bodies. PsGEF genetically interacts with Rac1 but not other Rho family members, and the RhoGEF domain of PsGEF induces actin polymerization in the membrane, thus resulting in the membrane ruffling that is observed in cultured cells with activated forms of Rac.
CONCLUSION:The specific acquisition of PsGEF by the last common ancestor of protostomes followed by its retention or loss in specific animal species during evolution demonstrates that there are some structural and/or functional features common between insect and lophotrochozoan nervous systems (for example, mushroom bodies), which are absent in all deuterostomes and cnidarians. PsGEF is therefore one of genes associated with the diversity of metazoan nervous systems.
Volume 7
Pages 21
Published 2009-5-7
DOI 10.1186/1741-7007-7-21
PII 1741-7007-7-21
PMID 19422675
PMC PMC2684095
MeSH Amino Acid Sequence Animals Annelida / anatomy & histology* Axons / metabolism Biological Evolution* Brain / anatomy & histology* Brain / embryology Brain / metabolism Cells, Cultured Daphnia / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / metabolism Exons / genetics Gene Deletion Gene Expression Regulation, Developmental Guanine Nucleotide Exchange Factors / chemistry Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism* HeLa Cells Humans Insecta / anatomy & histology* Introns / genetics Molecular Sequence Data Mushroom Bodies / anatomy & histology* Organ Specificity Protein Structure, Tertiary RNA, Messenger / genetics RNA, Messenger / metabolism rac1 GTP-Binding Protein / metabolism rho GTP-Binding Proteins / metabolism
IF 5.77
Times Cited 2