| RRC ID |
6032
|
| 著者 |
Tambe Y, Yamamoto A, Isono T, Chano T, Fukuda M, Inoue H.
|
| タイトル |
The drs tumor suppressor is involved in the maturation process of autophagy induced by low serum.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
The drs gene is an apoptosis-inducing tumor suppressor. Previously, we showed that drs contributes to the suppression of tumor formation by generating drs-knockout mice. In this study, by using drs KO mouse embryonic fibroblasts, we found that drs is involved in the autophagy regulation under low serum culture conditions. Both electron microscopy and GFP-LC3 analyses demonstrated that drs is involved in the maturation process of autophagy from autophagosomes to autolysosomes. In addition, drs could associate with Rab24 and the association between drs and Rab24 was enhanced during autophagy. Drs was also co-localized with Rab24 on punctuated structures during autophagy.
|
| 巻・号 |
283(1)
|
| ページ |
74-83
|
| 公開日 |
2009-9-28
|
| DOI |
10.1016/j.canlet.2009.03.028
|
| PII |
S0304-3835(09)00220-1
|
| PMID |
19368996
|
| MeSH |
Animals
Autophagy / physiology*
Cell Culture Techniques
Cells, Cultured
DNA Fingerprinting
Fibroblasts / metabolism*
Fluorescent Antibody Technique
Genes, Tumor Suppressor / physiology*
Humans
Immunoblotting
Immunoprecipitation
Male
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mice
Mice, Knockout
Microscopy, Electron, Transmission
Phagosomes / genetics
Phagosomes / metabolism*
Serum
rab GTP-Binding Proteins / metabolism
|
| IF |
7.36
|
| 引用数 |
14
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00806 |
