論文 - 詳細
| RRC ID | 65267 |
|---|---|
| 著者 | Yan H, Chen C, Chen H, Hong H, Huang Y, Ling K, Hu J, Wei Q. |
| タイトル | TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating. |
| ジャーナル | Nat Commun |
| Abstract |
Transition fibers (TFs) regulate cilia gating and make the primary cilium a distinct functional entity. However, molecular insights into the biogenesis of a functional cilia gate remain elusive. In a forward genetic screen in Caenorhabditis elegans, we uncover that TALP-3, a homolog of the Joubert syndrome protein TALPID3, is a TF-associated component. Genetic analysis reveals that TALP-3 coordinates with ANKR-26, the homolog of ANKRD26, to orchestrate proper cilia gating. Mechanistically, TALP-3 and ANKR-26 form a complex with key gating component DYF-19, the homolog of FBF1. Co-depletion of TALP-3 and ANKR-26 specifically impairs the recruitment of DYF-19 to TFs. Interestingly, in mammalian cells, TALPID3 and ANKRD26 also play a conserved role in coordinating the recruitment of FBF1 to TFs. We thus report a conserved protein module that specifically regulates the functional component of the ciliary gate and suggest a correlation between defective gating and ciliopathy pathogenesis. |
| 巻・号 | 11(1) |
| ページ | 2196 |
| 公開日 | 2020-5-4 |
| DOI | 10.1038/s41467-020-16042-w |
| PII | 10.1038/s41467-020-16042-w |
| PMID | 32366837 |
| PMC | PMC7198521 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Animals, Genetically Modified Basal Bodies / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cells, Cultured Centrioles / metabolism Cilia / genetics Cilia / metabolism* HEK293 Cells Humans Microscopy, Confocal Mutation RNA Interference Retinal Pigment Epithelium / cytology Retinal Pigment Epithelium / metabolism |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3067 tm7883 |