論文 - 詳細
RRC ID | 6594 |
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著者 | Ohta T, Ikemoto Y, Saeki K, Koide T, Wakabayashi S. |
タイトル | Histidine-rich glycoprotein and concanavalin A synergistically stimulate the phosphatidylinositol 3-kinase-independent signaling pathway in leukocytes leading to increased cell adhesion and changes in cell morphology. |
ジャーナル | Cell Immunol |
Abstract |
Histidine-rich glycoprotein (HRG) promoted the adhesion and morphological changes of human T-cell line MOLT-4 in a Con A-dependent manner. This morphological change-promoting activity was specific for HRG and the Arg23-Lys66 glycopeptide from human HRG. The carbohydrate chain at Asn45 was essential for this activity. The morphological changes of MOLT-4 cells caused by HRG and Con A (HRG/Con A) were not inhibited by phosphatidylinositol 3-kinase inhibitor, wortmannin or LY294002, while the changes by Con A alone were completely inhibited by these reagents, suggesting that HRG/Con A cooperate to activate leukocytes via a signaling pathway distinct from that by Con A alone. The morphological changes by Con A were associated with pseudopodia like structure. On the other hand, the morphological changes caused by HRG/Con A were associated not only with pseudopodia like structure but also with an increase of the F-actin-rich surface protrusions. Wortmannin inhibited only the formation of pseudopodia like structure. |
巻・号 | 259(1) |
ページ | 5-12 |
公開日 | 2009-1-1 |
DOI | 10.1016/j.cellimm.2009.05.001 |
PII | S0008-8749(09)00080-X |
PMID | 19535045 |
MeSH | Androstadienes / pharmacology Anticoagulants / pharmacology Cell Adhesion / drug effects Cell Adhesion / immunology Chromones / pharmacology Concanavalin A / metabolism* Concanavalin A / pharmacology Enzyme Inhibitors / pharmacology Heparin / pharmacology Humans Leukocytes / drug effects Leukocytes / immunology* Mitogens / pharmacology Morpholines / pharmacology Phosphatidylinositol 3-Kinases / metabolism* Phosphoinositide-3 Kinase Inhibitors Protein Kinase Inhibitors / pharmacology Proteins / metabolism* Proteins / pharmacology Pseudopodia / drug effects Pseudopodia / physiology Signal Transduction / drug effects Wortmannin |
IF | 4.078 |
引用数 | 5 |
WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | MOLT-4(RCB0206) |