RRC ID 6594
著者 Ohta T, Ikemoto Y, Saeki K, Koide T, Wakabayashi S.
タイトル Histidine-rich glycoprotein and concanavalin A synergistically stimulate the phosphatidylinositol 3-kinase-independent signaling pathway in leukocytes leading to increased cell adhesion and changes in cell morphology.
ジャーナル Cell Immunol
Abstract Histidine-rich glycoprotein (HRG) promoted the adhesion and morphological changes of human T-cell line MOLT-4 in a Con A-dependent manner. This morphological change-promoting activity was specific for HRG and the Arg23-Lys66 glycopeptide from human HRG. The carbohydrate chain at Asn45 was essential for this activity. The morphological changes of MOLT-4 cells caused by HRG and Con A (HRG/Con A) were not inhibited by phosphatidylinositol 3-kinase inhibitor, wortmannin or LY294002, while the changes by Con A alone were completely inhibited by these reagents, suggesting that HRG/Con A cooperate to activate leukocytes via a signaling pathway distinct from that by Con A alone. The morphological changes by Con A were associated with pseudopodia like structure. On the other hand, the morphological changes caused by HRG/Con A were associated not only with pseudopodia like structure but also with an increase of the F-actin-rich surface protrusions. Wortmannin inhibited only the formation of pseudopodia like structure.
巻・号 259(1)
ページ 5-12
公開日 2009-1-1
DOI 10.1016/j.cellimm.2009.05.001
PII S0008-8749(09)00080-X
PMID 19535045
MeSH Androstadienes / pharmacology Anticoagulants / pharmacology Cell Adhesion / drug effects Cell Adhesion / immunology Chromones / pharmacology Concanavalin A / metabolism* Concanavalin A / pharmacology Enzyme Inhibitors / pharmacology Heparin / pharmacology Humans Leukocytes / drug effects Leukocytes / immunology* Mitogens / pharmacology Morpholines / pharmacology Phosphatidylinositol 3-Kinases / metabolism* Phosphoinositide-3 Kinase Inhibitors Protein Kinase Inhibitors / pharmacology Proteins / metabolism* Proteins / pharmacology Pseudopodia / drug effects Pseudopodia / physiology Signal Transduction / drug effects Wortmannin
IF 4.078
引用数 5
WOS 分野 IMMUNOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 MOLT-4(RCB0206)