| RRC ID |
6811
|
| 著者 |
Wang L, Nishihara H, Kimura T, Kato Y, Tanino M, Nishio M, Obara M, Endo T, Koike T, Tanaka S.
|
| タイトル |
DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.
|
| 巻・号 |
395(1)
|
| ページ |
111-5
|
| 公開日 |
2010-4-23
|
| DOI |
10.1016/j.bbrc.2010.03.148
|
| PII |
S0006-291X(10)00623-6
|
| PMID |
20350533
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation
Enzyme Activation
Extracellular Signal-Regulated MAP Kinases / metabolism*
GTPase-Activating Proteins
Gene Knockdown Techniques
Guanine Nucleotide Exchange Factors / genetics
Guanine Nucleotide Exchange Factors / metabolism*
Humans
Lymphoma, B-Cell / genetics
Lymphoma, B-Cell / pathology*
Mice
Proto-Oncogene Proteins c-akt / metabolism*
RNA, Small Interfering / genetics
Xenograft Model Antitumor Assays
|
| IF |
2.985
|
| 引用数 |
15
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
