RRC ID 711
Author Morimoto R, Kosugi T, Nakamura C, Takumi S.
Title Intragenic diversity and functional conservation of the three homoeologous loci of the KN1-type homeobox gene Wknox1 in common wheat.
Journal Plant Mol. Biol.
Abstract Common wheat represents a typical allohexaploid species and provides a good experimental system for studying genomic alterations associated with allopolyploidization. We studied three homoeologous loci of wheat Wknox1 gene, which is highly homologous to the knotted1 (kn1)-like homeobox (KNOX) genes functioning at shoot apical meristems (SAM). Wknox1 transcripts were detected in SAM, and its overexpression caused abnormal leaf morphology with occasional ectopic leaves in transgenic tobacco plants. A comparative study of the three Wknox1 genomic sequences revealed accumulation of a large number of mutations including insertions and deletions, particularly in the fourth intron and the 5'-upstream region. Some structural mutations including MITE-insertions were allocated in the evolutionary lineage of the wheat genome, suggesting that they occurred at all stages of wheat evolution. A mutation rate was the highest in the Wknox1b locus, which is consistent with the known highest degree of differentiation in the B genome. Despite the structural differentiation, all three Wknox1 homoeologs showed an identical expression pattern in wheat and their promoter regions induced the conserved expression pattern in transgenic tobacco plants. A potential of the intragenic diversity in homoeologs of essential genes as a tool for studying the genome evolution associated with allopolyploidization was discussed.
Volume 57(6)
Pages 907-24
Published 2005-4
DOI 10.1007/s11103-005-3247-2
PMID 15952073
MeSH 5' Flanking Region / genetics Base Sequence Cloning, Molecular DNA Transposable Elements / genetics DNA, Plant / chemistry DNA, Plant / genetics* Evolution, Molecular Exons / genetics Gene Expression Profiling Genes, Plant / genetics Genetic Variation Genome, Plant Glucuronidase / genetics Glucuronidase / metabolism Homeodomain Proteins / genetics* Homeodomain Proteins / physiology Introns / genetics Molecular Sequence Data Mutagenesis, Insertional Mutation Plant Proteins / genetics* Plant Proteins / physiology Plants, Genetically Modified Polymorphism, Single Nucleotide Polyploidy Protein Isoforms / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Analysis, DNA Sequence Deletion Sequence Homology, Nucleic Acid Triticum / genetics* Triticum / physiology
IF 3.543
Times Cited 18
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Wheat KU-199-11 KU-105 KU-3636 KU-101-2 KU-101-3 KU-103 KU-1-2 KU-1-3 KU-2-4 KU-2-5 KU-3-2 KU-3-3 KU-4-5 KU-4-6 KU-4-7 KU-5-2 KU-5-3 KU-20-1 KU-20-3 KU-20-6 etc