RRC ID 77370
Author Ueda T, Hanai A, Takei T, Kubo K, Ohgi M, Sakagami H, Takahashi S, Shin HW, Nakayama K.
Title EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis.
Journal FEBS Lett
Abstract The small GTPase Arf6 is transiently associated with the ingressing cleavage furrow and subsequently targeted to the Flemming body during cytokinesis, suggesting its activation around the cleavage furrow. Here, we show that EFA6 (exchange factor for Arf6) localizes on the cleavage furrow through its PH domain. Time-lapse analysis showed that both EFA6 and Arf6 are transiently localized around the ingressing cleavage furrow, but only Arf6 is subsequently targeted to the Flemming body. Expression of an EFA6 mutant suppresses Arf6 recruitment onto the Flemming body. These results suggest that EFA6 participates in activation of Arf6 around the cleavage furrow during cytokinesis.
Volume 587(11)
Pages 1617-23
Published 2013-6-5
DOI 10.1016/j.febslet.2013.03.042
PII S0014-5793(13)00298-6
PMID 23603394
MeSH ADP-Ribosylation Factor 6 ADP-Ribosylation Factors / metabolism* Amino Acid Sequence Amino Acid Substitution Conserved Sequence Cytokinesis* Enzyme Activation Guanine Nucleotide Exchange Factors HeLa Cells Humans Microscopy, Fluorescence Mutagenesis, Site-Directed Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Protein Structure, Tertiary Protein Transport Single-Cell Analysis Time-Lapse Imaging
IF 3.057
DNA material EGFP-hEFA6A (RDB20108) EGFP-hEFA6B (RDB20109) EGFP-hEFA6C (RDB20110)