論文 - 詳細
| RRC ID | 11411 |
|---|---|
| 著者 | Sirichandra C, Davanture M, Turk BE, Zivy M, Valot B, Leung J, Merlot S. |
| タイトル | The Arabidopsis ABA-activated kinase OST1 phosphorylates the bZIP transcription factor ABF3 and creates a 14-3-3 binding site involved in its turnover. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Genetic evidence in Arabidopsis thaliana indicates that members of the Snf1-Related Kinases 2 family (SnRK2) are essential in mediating various stress-adaptive responses. Recent reports have indeed shown that one particular member, Open Stomata (OST)1, whose kinase activity is stimulated by the stress hormone abscisic acid (ABA), is a direct target of negative regulation by the core ABA co-receptor complex composed of PYR/PYL/RCAR and clade A Protein Phosphatase 2C (PP2C) proteins. METHODOLOGY/PRINCIPAL FINDINGS:Here, the substrate preference of OST1 was interrogated at a genome-wide scale. We phosphorylated in vitro a bank of semi-degenerate peptides designed to assess the relative phosphorylation efficiency on a positionally fixed serine or threonine caused by systematic changes in the flanking amino acid sequence. Our results designate the ABA-responsive-element Binding Factor 3 (ABF3), which controls part of the ABA-regulated transcriptome, as a genuine OST1 substrate. Bimolecular Fluorescence Complementation experiments indicate that ABF3 interacts directly with OST1 in the nuclei of living plant cells. In vitro, OST1 phosphorylates ABF3 on multiple LXRXXpS/T preferred motifs including T451 located in the midst of a conserved 14-3-3 binding site. Using an antibody sensitive to the phosphorylated state of the preferred motif, we further show that ABF3 is phosphorylated on at least one such motif in response to ABA in vivo and that phospho-T451 is important for stabilization of ABF3. CONCLUSIONS/SIGNIFICANCE:All together, our results suggest that OST1 phosphorylates ABF3 in vivo on T451 to create a 14-3-3 binding motif. In a wider physiological context, we propose that the long term responses to ABA that require sustained gene expression is, in part, mediated by the stabilization of ABFs driven by ABA-activated SnRK2s. |
| 巻・号 | 5(11) |
| ページ | e13935 |
| 公開日 | 2010-11-10 |
| DOI | 10.1371/journal.pone.0013935 |
| PMID | 21085673 |
| PMC | PMC2978106 |
| MeSH | 14-3-3 Proteins / genetics 14-3-3 Proteins / metabolism* Abscisic Acid / pharmacology Amino Acid Motifs / genetics Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism* Binding Sites / genetics Cell Nucleus / metabolism Chromatography, Liquid Gene Expression Profiling Gene Expression Regulation, Plant Immunoblotting Mass Spectrometry Molecular Sequence Data Mutation Phosphorylation / drug effects Plant Growth Regulators / pharmacology Plants, Genetically Modified Protein Binding / drug effects Protein Kinases / genetics Protein Kinases / metabolism* Protein Stability / drug effects Reverse Transcriptase Polymerase Chain Reaction Substrate Specificity rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism |
| IF | 2.74 |
| 引用数 | 119 |
| WOS 分野 | PLANT SCIENCES |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda14224 |