RRC ID 2049
Author Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H.
Title Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
Journal J Neurochem
Abstract Axotomy-induced neuronal death occurs in neonatal motoneurons, but not in adult rat. Here we demonstrated that during the course of postnatal development, nerve injury induced down-regulation of the glial cell line-derived neurotrophic factor (GDNF) receptor GFRalpha1 in axotomized hypoglossal motoneurons of rat are gradually converted to the adult up-regulation pattern of response. The compensatory expression of GFRalpha1 specifically in the injured motoneurons of neonates by adenovirus succeeded in rescuing the injured neurons without an application of growth factors. To the contrary, the nuclear antisense RNA for GFRalpha1 expression accelerates the axotomy-induced neuronal death in pups. These findings suggest that the receptor expression response after nerve injury is critical for the determination of injured motoneuron fate.
Volume 82(4)
Pages 961-75
Published 2002-8-1
DOI 10.1046/j.1471-4159.2002.01043.x
PII 1043
PMID 12358802
MeSH Age Factors Animals Animals, Newborn Axotomy Brain-Derived Neurotrophic Factor / pharmacology Cell Death / drug effects Cell Line Down-Regulation Drosophila Proteins* Gene Expression Regulation, Developmental* / drug effects Genetic Vectors / administration & dosage Genetic Vectors / genetics Glial Cell Line-Derived Neurotrophic Factor Glial Cell Line-Derived Neurotrophic Factor Receptors Humans Hypoglossal Nerve / growth & development Hypoglossal Nerve / metabolism* Hypoglossal Nerve Injuries* Immunohistochemistry In Situ Hybridization Membrane Glycoproteins* Mononeuropathies / metabolism* Motor Neurons / cytology Motor Neurons / drug effects Motor Neurons / metabolism* Nerve Growth Factors* Nerve Tissue Proteins / pharmacology Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins / pharmacology Proto-Oncogene Proteins c-ret RNA, Antisense / pharmacology RNA, Messenger / antagonists & inhibitors RNA, Messenger / metabolism Rats Rats, Wistar Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism* Receptor Protein-Tyrosine Kinases / pharmacology Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Receptors, Nerve Growth Factor* Up-Regulation
IF 4.87
Times Cited 23
DNA material pCALwL (RDB01679)