論文 - 詳細
| RRC ID | 22701 |
|---|---|
| 著者 | Zhang Z, Lv X, Yin WC, Zhang X, Feng J, Wu W, Hui CC, Zhang L, Zhao Y. |
| タイトル | Ter94 ATPase complex targets k11-linked ubiquitinated ci to proteasomes for partial degradation. |
| ジャーナル | Dev Cell |
| Abstract |
The Cubitus interruptus (Ci)/Gli family of transcription factors can be degraded either completely or partially from a full-length form (Ci155/Gli(FL)) to a truncated repressor (Ci75/Gli(R)) by proteasomes to mediate Hedgehog (Hh) signaling. The mechanism by which proteasomes distinguish ubiquitinated Ci/Gli to carry out complete versus partial degradation is not known. Here, we show that Ter94 ATPase and its mammalian counterpart, p97, are involved in processing Ci and Gli3 into Ci75 and Gli3(R), respectively. Ter94 regulates the partial degradation of ubiquitinated Ci by Cul1-Slimb-based E3 ligase through its adaptors Ufd1-like and dNpl4. We demonstrate that Cul1-Slimb-based E3 ligase, but not Cul3-Rdx-based E3 ligase, modifies Ci by efficient addition of K11-linked ubiquitin chains. Ter94(Ufd1-like/dNpl4) complex interacts directly with Cul1-Slimb, and, intriguingly, it prefers K11-linked ubiquitinated Ci. Thus, Ter94 ATPase and K11-linked ubiquitination in Ci contribute to the selectivity by proteasomes for partial degradation. |
| 巻・号 | 25(6) |
| ページ | 636-44 |
| 公開日 | 2013-6-24 |
| DOI | 10.1016/j.devcel.2013.05.006 |
| PII | S1534-5807(13)00280-3 |
| PMID | 23747190 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Carrier Proteins / genetics Carrier Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cells, Cultured Cullin Proteins / genetics Cullin Proteins / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Hedgehog Proteins / genetics Hedgehog Proteins / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / metabolism* Protein Binding / physiology Signal Transduction / physiology Smad Proteins, Inhibitory / genetics Transcription Factors / genetics Transcription Factors / metabolism* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism Ubiquitination / physiology Valosin Containing Protein |
| IF | 10.092 |
| 引用数 | 33 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 6233R-1 4673R-2 |