RRC ID 28693
Author Smialowska A, Djupedal I, Wang J, Kylsten P, Swoboda P, Ekwall K.
Title RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe.
Journal Biochem Biophys Res Commun
Abstract RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.
Volume 444(2)
Pages 254-9
Published 2014-2-7
DOI 10.1016/j.bbrc.2014.01.057
PII S0006-291X(14)00081-3
PMID 24462781
MeSH Argonaute Proteins / genetics Argonaute Proteins / metabolism Blotting, Northern Blotting, Western Endoribonucleases / genetics Endoribonucleases / metabolism Gene Expression Regulation, Fungal* Microscopy, Fluorescence Mutation Oligonucleotide Array Sequence Analysis RNA Interference* RNA, Antisense / genetics RNA, Fungal / genetics RNA, Messenger / genetics RNA, Small Interfering / genetics RNA, Small Untranslated / genetics RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Transcriptome / genetics*
IF 2.985
Times Cited 11
DNA material S. pombe YFP-FLAG-His SpYFH23B11 (SPW089235) SpYFH22C07 (SPW088855) SpYFH46F08 (SPW098528) SpYFH40H10 (SPW096178) SpYFH38C10 (SPW095258) SpYFH04F01 (SPW081721) SpYFH39B05 (SPW095629) SpYFH31D08 (SPW092480).