RRC ID 30465
Author Rolls MM, Satoh D, Clyne PJ, Henner AL, Uemura T, Doe CQ.
Title Polarity and intracellular compartmentalization of Drosophila neurons.
Journal Neural Dev
Abstract BACKGROUND:Proper neuronal function depends on forming three primary subcellular compartments: axons, dendrites, and soma. Each compartment has a specialized function (the axon to send information, dendrites to receive information, and the soma is where most cellular components are produced). In mammalian neurons, each primary compartment has distinctive molecular and morphological features, as well as smaller domains, such as the axon initial segment, that have more specialized functions. How neuronal subcellular compartments are established and maintained is not well understood. Genetic studies in Drosophila have provided insight into other areas of neurobiology, but it is not known whether flies are a good system in which to study neuronal polarity as a comprehensive analysis of Drosophila neuronal subcellular organization has not been performed.
RESULTS:Here we use new and previously characterized markers to examine Drosophila neuronal compartments. We find that: axons and dendrites can accumulate different microtubule-binding proteins; protein synthesis machinery is concentrated in the cell body; pre- and post-synaptic sites localize to distinct regions of the neuron; and specializations similar to the initial segment are present. In addition, we track EB1-GFP dynamics and determine microtubules in axons and dendrites have opposite polarity.
CONCLUSION:We conclude that Drosophila will be a powerful system to study the establishment and maintenance of neuronal compartments.
Volume 2
Pages 7
Published 2007-4-30
DOI 10.1186/1749-8104-2-7
PII 1749-8104-2-7
PMID 17470283
PMC PMC1868948
MeSH Animals Axons / metabolism Axons / ultrastructure Brain / cytology* Brain / growth & development Brain / metabolism Cell Compartmentation / physiology* Cell Differentiation / physiology* Cell Polarity / physiology* Dendrites / metabolism Dendrites / ultrastructure Drosophila melanogaster / cytology* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Green Fluorescent Proteins / metabolism Larva / cytology Larva / growth & development Larva / metabolism Microtubule-Associated Proteins / biosynthesis Microtubules / metabolism Microtubules / ultrastructure Models, Animal Mushroom Bodies / cytology Mushroom Bodies / growth & development Mushroom Bodies / metabolism Neurons / cytology* Neurons / metabolism Protein Transport / physiology Recombinant Fusion Proteins / metabolism
IF 2.63
Times Cited 108
WOS Category NEUROSCIENCES DEVELOPMENTAL BIOLOGY
Resource
Drosophila DGRC#109613