論文 - 詳細
| RRC ID | 42770 |
|---|---|
| 著者 | Shinozaki-Narikawa N, Kodama T, Shibasaki Y. |
| タイトル | Cooperation of phosphoinositides and BAR domain proteins in endosomal tubulation. |
| ジャーナル | Traffic |
| Abstract |
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate many intracellular events, including vesicular trafficking and actin remodeling, by recruiting proteins to their sites of function. PtdIns(4,5)-bisphosphate [PI(4,5)P2] and related phosphoinositides are mainly synthesized by type I PtdIns-4-phosphate 5-kinases (PIP5Ks). We found that PIP5K induces endosomal tubules in COS-7 cells. ADP-ribosylation factor (ARF) 6 has been shown to act upstream of PIP5K and regulate endocytic transport and tubulation. ARF GAP with coiled-coil, ankyrin repeat, and pleckstrin homology domains 1 (ACAP1) has guanosine triphosphatase-activating protein (GAP) activity for ARF6. While there were few tubules induced by the expression of ACAP1 alone, numerous endosomal tubules were induced by coexpression of PIP5K and ACAP1. ACAP1 has a pleckstrin homology (PH) domain known to bind phosphoinositide and a Bin/amphiphysin/Rvs (BAR) domain that has been reported to detect membrane curvature. Truncated and point mutations in the ACAP1 BAR and PH domains revealed that both BAR and PH domains are required for tubulation. These results suggest that two ARF6 downstream molecules, PIP5K and ACAP1, function together in endosomal tubulation and that phosphoinositide levels may regulate endosomal dynamics. |
| 巻・号 | 7(11) |
| ページ | 1539-50 |
| 公開日 | 2006-11-1 |
| DOI | 10.1111/j.1600-0854.2006.00480.x |
| PII | TRA480 |
| PMID | 17010122 |
| MeSH | ADP-Ribosylation Factor 6 Animals Binding Sites / genetics COS Cells Chlorocebus aethiops Endosomes / drug effects Endosomes / metabolism* GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism* Humans Isoenzymes / genetics Isoenzymes / metabolism Mice Microtubules / drug effects Microtubules / metabolism Mutation / genetics Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nocodazole / pharmacology Phosphatidylinositol 4,5-Diphosphate / metabolism Phosphatidylinositols / metabolism* Phospholipase C delta Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism* Protein Binding Recombinant Fusion Proteins / metabolism Transfection Tubulin / metabolism Type C Phospholipases / genetics Type C Phospholipases / metabolism |
| IF | 4.045 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |