論文 - 詳細
| RRC ID | 438 |
|---|---|
| 著者 | Schumacher B, Schertel C, Wittenburg N, Tuck S, Mitani S, Gartner A, Conradt B, Shaham S. |
| タイトル | C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage. |
| ジャーナル | Cell Death Differ |
| Abstract |
The p53 tumor suppressor promotes apoptosis in response to DNA damage. Here we describe the Caenorhabditis elegans gene ced-13, which encodes a conserved BH3-only protein. We show that ced-13 mRNA accumulates following DNA damage, and that this accumulation is dependent on an intact C. elegans cep-1/p53 gene. We demonstrate that CED-13 protein physically interacts with the antiapoptotic Bcl-2-related protein CED-9. Furthermore, overexpression of ced-13 in somatic cells leads to the death of cells that normally survive, and this death requires the core apoptotic pathway of C. elegans. Recent studies have implicated two BH3-only proteins, Noxa and PUMA, in p53-induced apoptosis in mammals. Our studies suggest that in addition to the BH3-only protein EGL-1, CED-13 might also promote apoptosis in the C. elegans germ line in response to p53 activation. We propose that an evolutionarily conserved pathway exists in which p53 promotes cell death by inducing expression of two BH3-only genes. |
| 巻・号 | 12(2) |
| ページ | 153-61 |
| 公開日 | 2005-2-1 |
| DOI | 10.1038/sj.cdd.4401539 |
| PII | 4401539 |
| PMID | 15605074 |
| MeSH | Amino Acid Sequence Animals Apoptosis / physiology* Apoptosis Regulatory Proteins Caenorhabditis / genetics Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA / genetics DNA / radiation effects DNA Damage* Gene Deletion Gene Expression Regulation, Developmental / radiation effects Heat-Shock Proteins / genetics Models, Biological Molecular Sequence Data Mutation Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2 Repressor Proteins / genetics Sequence Homology, Amino Acid Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / physiology X-Rays |
| IF | 10.717 |
| 引用数 | 121 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm536 tm586 |