論文 - 詳細
| RRC ID | 45639 |
|---|---|
| 著者 | Sallee MD, Greenwald I. |
| タイトル | Dimerization-driven degradation of C. elegans and human E proteins. |
| ジャーナル | Genes Dev |
| Abstract |
E proteins are conserved regulators of growth and development. We show that the Caenorhabditis elegans E-protein helix-loop-helix-2 (HLH-2) functions as a homodimer in directing development and function of the anchor cell (AC) of the gonad, the critical organizer of uterine and vulval development. Our structure-function analysis of HLH-2 indicates that dimerization drives its degradation in other uterine cells (ventral uterine precursor cells [VUs]) that initially have potential to be the AC. We also provide evidence that this mode of dimerization-driven down-regulation can target other basic HLH (bHLH) dimers as well. Remarkably, human E proteins can functionally substitute for C. elegans HLH-2 in regulating AC development and also display dimerization-dependent degradation in VUs. Our results suggest that dimerization-driven regulation of bHLH protein stability may be a conserved mechanism for differential regulation in specific cell contexts. |
| 巻・号 | 29(13) |
| ページ | 1356-61 |
| 公開日 | 2015-7-1 |
| DOI | 10.1101/gad.261917.115 |
| PII | 29/13/1356 |
| PMID | 26159995 |
| PMC | PMC4511211 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism* Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / metabolism Cells, Cultured Dimerization Down-Regulation Female Gene Expression Regulation, Developmental* Humans Protein Processing, Post-Translational* Protein Stability Proteolysis* Uterus / embryology |
| IF | 9.527 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1768 |