論文 - 詳細
| RRC ID | 45916 |
|---|---|
| 著者 | Wu Y, Cheng S, Zhao H, Zou W, Yoshina S, Mitani S, Zhang H, Wang X. |
| タイトル | PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation. |
| ジャーナル | EMBO Rep |
| Abstract |
Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphatase MTM-3 catalyzes PtdIns3P turnover late in autophagy. MTM-3 acts downstream of the ATG-2/EPG-6 complex and upstream of EPG-5 to promote autophagosome maturation into autolysosomes. MTM-3 is recruited to autophagosomes by PtdIns3P, and loss of MTM-3 causes increased autophagic association of ATG-18 in a PtdIns3P-dependent manner. Our data reveal critical roles of PtdIns3P turnover in autophagosome maturation and/or autolysosome formation. |
| 巻・号 | 15(9) |
| ページ | 973-81 |
| 公開日 | 2014-9-1 |
| DOI | 10.15252/embr.201438618 |
| PII | embr.201438618 |
| PMID | 25124690 |
| PMC | PMC4198041 |
| MeSH | Animals Autophagy / genetics* Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism Lysosomes / genetics Lysosomes / metabolism Phagosomes / genetics* Phosphatidylinositol 3-Kinases / genetics Phosphatidylinositol 3-Kinases / metabolism* Phosphatidylinositol Phosphates / metabolism* Phosphoric Monoester Hydrolases / metabolism Protein Tyrosine Phosphatases, Non-Receptor / metabolism |
| IF | 7.497 |
| 引用数 | 26 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4475 |