論文 - 詳細
| RRC ID | 45988 |
|---|---|
| 著者 | Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. |
| タイトル | DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. |
| ジャーナル | Dev Cell |
| Abstract |
Developmental timing genes catalyze stem cell progression and animal maturation programs across taxa. Caenorhabditis elegans DRE-1/FBXO11 functions in an SCF E3-ubiquitin ligase complex to regulate the transition to adult programs, but its cognate proteolytic substrates are unknown. Here, we identify the conserved transcription factor BLMP-1 as a substrate of the SCF(DRE-1/FBXO11) complex. blmp-1 deletion suppressed dre-1 mutant phenotypes and exhibited developmental timing defects opposite to dre-1. blmp-1 also opposed dre-1 for other life history traits, including entry into the dauer diapause and longevity. BLMP-1 protein was strikingly elevated upon dre-1 depletion and dysregulated in a stage- and tissue-specific manner. The role of DRE-1 in regulating BLMP-1 stability is evolutionary conserved, as we observed direct protein interaction and degradation function for worm and human counterparts. Taken together, posttranslational regulation of BLMP-1/BLIMP-1 by DRE-1/FBXO11 coordinates C. elegans developmental timing and other life history traits, suggesting that this two-protein module mediates metazoan maturation processes. |
| 巻・号 | 28(6) |
| ページ | 697-710 |
| 公開日 | 2014-3-31 |
| DOI | 10.1016/j.devcel.2014.01.028 |
| PII | S1534-5807(14)00069-0 |
| PMID | 24613396 |
| PMC | PMC4040421 |
| MeSH | Animals Blotting, Western Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Epidermal Cells Epidermis / metabolism F-Box Proteins / antagonists & inhibitors F-Box Proteins / genetics F-Box Proteins / metabolism* Gene Expression Regulation, Developmental* Gene Silencing Gonads / cytology Gonads / metabolism HEK293 Cells Humans Immunoenzyme Techniques Larva / cytology* Larva / metabolism Longevity / genetics* Organ Specificity Proteasome Endopeptidase Complex / metabolism Proteolysis RNA, Small Interfering / genetics Time Factors Transcription Factors / genetics Transcription Factors / metabolism Ubiquitination |
| IF | 10.092 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm548 |